Tgf β1 single plex magnetic bead kit

A list of the 33 proteins assayed and known primary function is shown in Table 1. A Biorad protein assay was run for each tissue type (plasma and intestine) and experimental groups (For example, 19.75 Gy WAI, 19.75 Gy WAI + LR, 19.75 Gy WAI + IL-22, and 19.75 Gy WAI + LR-IL-22) using a Biotek Epoch microplate-spectrophotometer (Biotek Instruments, Inc., Winooski, VT). We utilized a TGF-β1 Single Plex Magnetic Bead Kit, as well as a 32 Multiplex Mouse Cytokine/Chemokine Magnetic Bead Panel (EMD Millipore, Billerica, MA, USA) that tested protein concentrations for each protein (Milliplex® for Luminex® xMAP® Technology). Each kit (MCYTOMAG-70K, TGFBMAG-64K-01) provided reagents for use in the Luminex Protein Immunoassay, which included 1 vial of Mouse Cytokine/Chemokine Standard, 2 vials Mouse Cytokine/Chemokine Quality Controls, 1 vial Serum Matrix, 96-Well Plate, 30 mL Assay Buffer, 60 mL 10X Wash Buffer, 3.2 mL bottle Mouse Cytokine Detection Antibodies, 1 3.2 mL bottle Streptavidin-Phycoerythrin. The 32 Multiplex kit (MCYTOMAG-70K) provided 1 3.5 mL pre-Mixed 32-plex Beads. The TGF-β1 kit (TGFBMAG-64K-01) provided the above reagents, as well as 5 mL Sample Diluent, 1.0 mL 1.0 N HCl, 1.0 mL 1 N NaOH, and 3.5 mL Anti-TGFB1 Beads.