2 1h 7 azabenzotriazole 1 yl 1 1 3 3 tetramethyl uronium hexafluorophosphate methanaminium hatu

The active compound Lico-A was obtained from Amadis Chemical (Hangzhou, China) and the polymer PLGA 50:50 Resomer^® RG 503 H, 34 KD, from Boehringer Ingelheim (Ingelheim, Germany). Maleimide-PEG-NH₂ (5KDa) was from Jenkem (Beijing, China). NovaSyn TGR resin and 9-fluorenyl-methoxycarbonyl (Fmoc) protected amino acids were purchased from Novabiochem (Merck Millipore, Merck KGaA, Darmstadt, Germany). Peptide-synthesis-grade dimethylformamide (DMF) and trifluroacetic acid (TFA) were obtained from Scharlau (Barcelona, Spain). HPLC-grade acetonitrile and acetone were purchased from Fisher Scientific (Loughborough, UK). The coupling reagent, 2-(1H-7-azabenzotriazole-1-yl)-1,1,3,3- tetra-methyluronium hexafluorophosphate methanaminium (HATU) was from Genscript (Piscataway, NJ, USA). Diisopropylethylamine (DIPEA), piperidine, triisopropylsilane (TIS), 2-mercaptoethanol, N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), dimethyl sulfoxide (DMSO), dimethyl sulfoxide-d6 (DMSO-d6), and sodium arachidonic acid (SA) were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Methanol and diethyl ether were purchased from Merck (KGaA, Darmstadt, Germany). Water filtered through the Millipore^® MilliQ system was used for all experiments and the other chemicals and reagents used in the study were of analytical grade.