Charcoal dextran treated fbs

The PKV cell line was generated by FACS sorting CD45^-CD31^-Ter119^-EpCAM⁺GFP^- epithelial cells from the prostates of 10 week old CPKV mice and culturing them in 0.2% gelatin-coated 10 cm dishes with Dulbecco’s modified eagle medium (DMEM) (Sigma-Aldrich, St. Louis, MO, USA) containing 1% Pen/Strep, 10% Fetal Bovine Serum (FBS) (Omega Scientific, Tarzana, CA, USA), 25 μg /mL bovine pituitary extract, 5 μg /mL insulin (Invitrogen, Grand Island, NY, USA), and 6 ng/mL recombinant human EGF (BD Biosciences, San Jose, CA, USA). LNCaP cells were grown in RPMI-1640 media (Sigma-Aldrich) with 1% Pen/Strep and 10% FBS. For studies carried out in the context of androgen deprivation, media containing 10% charcoal dextran-treated (CDT) FBS (BD Biosciences) was used.