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Dionex ultimate ncp 3200rs

Manufactured by Thermo Fisher Scientific
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Sourced in United States

The Dionex UltiMate NCP-3200RS is a robust and versatile nano-flow liquid chromatography system capable of delivering precise and reliable separations at nano-scale flow rates. It features a high-pressure gradient pump, an auto-sampler, and a column compartment for temperature control. The system is designed to support a wide range of applications in fields such as proteomics, metabolomics, and other life science research.

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Lab products found in correlation

Ethylene bridged hybrid c18, by Waters Corporation (1 mentions) C18 packing material, by Phenomenex (1 mentions) Q exactive plus orbitrap ms, by Thermo Fisher Scientific (1 mentions) Self pack picofrit column, by New Objective (1 mentions) 1200 series, by Agilent Technologies (1 mentions) Pal autosampler, by CTC Analytics (1 mentions)

2 protocols using dionex ultimate ncp 3200rs

1

Single-Cell LC-MS/MS Workflow

Cited 1 time
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The LC-MS/MS method used for single-cell analysis were described previously (17 (link)) with slight modifications. The SPE precolumn (100 μm i.d., 360 μm o.d., 4 cm long) was packed with 3-μm C18 packing material (300-Å pore size, Phenomenex, Torrence, CA) and LC column (50 μm i.d., 360 μm o.d., 30 cm long) were packed with 1.7 μm C18 particles (Bridged Ethylene-Hybrid C18, Waters, Milford, MA). The LC column was heated at 50 °C during separation. The flow rate of the LC separation was 100 nL/min using a nanoUPLC pump (Dionex UltiMate NCP-3200RS, Thermo Scientific). A linear 100-min gradient of 8–30% buffer B (0.1% formic acid in acetonitrile) was used for the LC separation. Then the LC column was washed by ramping buffer B to 45% in 20 min and then keeping at 90% in 5 min, and finally re-equilibrated with 2% buffer B for another 10 min. A Thermo Scientific Q Exactive Plus was used for the MCF10A cells and an Orbitrap Fusion Lumos Tribrid mass spectrometer was employed for AML cells. The parameters for AGC and ion injection times were listed in the supplemental Table S1.
Tsai C.F., Zhao R., Williams S.M., Moore R.J., Schultz K., Chrisler W.B., Pasa-Tolic L., Rodland K.D., Smith R.D., Shi T., Zhu Y, & Liu T. (2020). An Improved Boosting to Amplify Signal with Isobaric Labeling (iBASIL) Strategy for Precise Quantitative Single-cell Proteomics. Molecular & Cellular Proteomics : MCP, 19(5), 828-838.
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2

Peptide Profiling of Small Cell Samples

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Peptide digests from small numbers of cells were analyzed using a commonly available Q Exactive Plus Orbitrap MS (Thermo Scientific, San Jose, CA). The standard LC system consisted of a PAL autosampler (CTC ANALYTICS AG, Zwingen, Switzerland), two Cheminert six-port injection valves (Valco Instruments, Houston, USA), a binary nanoUPLC pump (Dionex UltiMate NCP-3200RS, Thermo Scientific), and an HPLC sample loading pump (1200 Series, Agilent, Santa Clara, USA). Both SPE precolumn (150 μm i.d., 4 cm length) and LC column (50 μm i.d., 70 cm Self-Pack PicoFrit column, New Objective, Woburn, USA) were slurry-packed with 3 μm C18 packing material (300 Å pore size) (Phenomenex, Terrence, USA). The sample was fully injected into a 20 μL loop and loaded onto the SPE column using buffer A (0.1% formic acid in water) at a flow rate of 5 μL/min for 20 min. Parameters for LC gradient and MS data acquisition have been previously described.20 (link)
Martin K., Zhang T., Lin T.T., Habowski A.N., Zhao R., Tsai C.F., Chrisler W.B., Sontag R.L., Orton D.J., Lu Y.J., Rodland K.D., Yang B., Liu T., Smith R.D., Qian W.J., Waterman M.L., Wiley H.S, & Shi T. (2021). Facile One-Pot Nanoproteomics for Label-Free Proteome Profiling of 50–1000 Mammalian Cells. Journal of proteome research, 20(9), 4452-4461.
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