Mouse tumour tissues were embedded into paraffin sections, treated for 2 h at 65°C and then deparaffinised. Before applying the primary antibodies at 4°C overnight, we carried out the antigen retrieval step. The slides were incubated with a secondary antibody for 2 h at 25°C and then conjugated to HRP (1:100; Zhongshanjinqiao, China). Liquid DAB+ Substrate (zsgb-bio, China) was used to detect HRP activity.
Liquid dab substrate
Manufactured by ZSGB-BIO
Sourced in China
Liquid DAB+ Substrate is a laboratory reagent used in immunohistochemistry and immunocytochemistry applications. It serves as a chromogenic substrate for the detection of target antigens labeled with horseradish peroxidase (HRP) enzyme conjugates. The substrate undergoes an enzymatic reaction with HRP, resulting in the formation of a brown-colored precipitate at the site of the target antigen.
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Lab products found in correlation
2 protocols using liquid dab substrate
1
Paraffin-Embedded Tissue Immunohistochemistry
2
Paraffin-Embedded Tissue Immunohistochemistry
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Tissues were embedded into paraffin sections and treated 2 hours at 65°C and then deparaffinised. Before applying the primary antibodies at 4°C overnight, we carried out the antigen retrieval step. After that, slides were incubated with secondary antibody about 2 hours at 25°C conjugated to HRP (1:100; Zhongshanjinqiao, China). The Liquid DAB+ Substrate (zsgb-bio, China) was used for detection of the HRP activity.
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