The serum levels of lactate dehydrogenase (LDH) were detected with a kit (A202-2, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) that was used according to the manufacturer's instructions. Briefly, samples (20 μL) were incubated with 5 μL distilled water and 25 μL matrix buffer for 15 min at 37°C and then incubated with 25 μL 2, 4-dinitrophenyl-hydrazone for another 15 min at 37°C. Next, NaOH (0.4 mol/L, 250 μL) was added, and the mixture was placed at room temperature for 5 min. Finally, OD values at the wavelength of 450 nm were measured with a microplate reader (Epoch, BioTek Instruments, Inc., Vermont, USA).
A202 2
Manufactured by Nanjing Jiancheng
Sourced in China
The A202-2 is a laboratory equipment product. It is designed for core laboratory functions but a detailed description cannot be provided while maintaining an unbiased and factual approach. Further information is not available.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using a202 2
1
Serum LDH Quantification Protocol
2
Lactic Acid and LDH Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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