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Evolve 512 camera

Manufactured by Nikon
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The Nikon Evolve 512 is a high-resolution camera designed for laboratory and scientific applications. It features a 12.2-megapixel CMOS sensor and can capture images at a resolution of 4,096 x 3,072 pixels. The camera supports a variety of imaging modes and provides a range of connectivity options for integration with external systems.

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Lab products found in correlation

Eclipse ti e, by Nikon (3 mentions) Cfi apo tirf 100 1.49 n a oil objective, by Nikon (3 mentions) Inubg2e zilcs, by Tokai Hit (3 mentions) Metamorph 7, by Molecular Devices (2 mentions) Photometrics evolve 512 emccd, by Teledyne (2 mentions) Optosplit 3 beamsplitter, by Cairn Research (2 mentions) Coolsnap hq2 ccd camera, by Teledyne (1 mentions) Coolsnap hq2, by Nikon (1 mentions)

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Evolve 512 EMCCD camera (2 citations)

3 protocols using evolve 512 camera

1

TIRF Microscopy for Live-Cell Imaging

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TIRFM was performed on an inverted research microscope Nikon Eclipse Ti-E (Nikon) with the perfect focus system (PFS) (Nikon), equipped with the Nikon CFI Apo TIRF 100× 1.49 N.A. oil objective (Nikon), Photometrics Evolve 512 EMCCD (Roper Scientific) and controlled with the MetaMorph 7.7 software (Molecular Devices). Images were projected onto the chip of Evolve 512 camera with intermediate lens 2.5X (Nikon C mount adapter 2.5X). To keep in vitro samples at 30°C, we used stage top incubator INUBG2E-ZILCS (Tokai Hit).
For excitation we used 491nm 100mW Stradus (Vortran), 561nm 100mW Jive (Cobolt) and 642 nm 110 mW Stradus (Vortran). We used ET-GFP 49002 filter set (Chroma) for imaging of proteins tagged with GFP, ET-mCherry 49008 filter set (Chroma) for imaging X-Rhodamine labelled tubulin or mCherry-EB3 and ET-405/488/561/647 for imaging SNAP-Alexa647. For simultaneous imaging of green and red fluorescence, we used the triple-band TIRF polychroic ZT405/488/561rpc (Chroma) and the triple-band laser emission filter ZET405/488/561m (Chroma), mounted in the metal cube (Chroma, 91032) together with Optosplit III beamsplitter (Cairn Research Ltd, UK) equipped with a double emission filter cube configured with ET525/50m, ET630/75m and T585LPXR (Chroma). We used sequential acquisition for triple colour imaging experiments.
Atherton J., Jiang K., Stangier M.M., Luo Y., Hua S., Houben K., van Hooff J.J., Joseph A.P., Scarabelli G., Grant B.J., Roberts A.J., Topf M., Steinmetz M.O., Baldus M., Moores C.A, & Akhmanova A. (2017). A structural model for microtubule minus-end recognition and protection by CAMSAP proteins. Nature structural & molecular biology, 24(11), 931-943.
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2

TIRF Microscopy for Live-Cell Imaging

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TIRFM was performed on an inverted research microscope Nikon Eclipse Ti-E (Nikon) with the perfect focus system (PFS) (Nikon), equipped with the Nikon CFI Apo TIRF 100× 1.49 N.A. oil objective (Nikon), Photometrics Evolve 512 EMCCD (Roper Scientific) and controlled with the MetaMorph 7.7 software (Molecular Devices). Images were projected onto the chip of Evolve 512 camera with intermediate lens 2.5X (Nikon C mount adapter 2.5X). To keep in vitro samples at 30°C, we used stage top incubator INUBG2E-ZILCS (Tokai Hit).
For excitation we used 491nm 100mW Stradus (Vortran), 561nm 100mW Jive (Cobolt) and 642 nm 110 mW Stradus (Vortran). We used ET-GFP 49002 filter set (Chroma) for imaging of proteins tagged with GFP, ET-mCherry 49008 filter set (Chroma) for imaging X-Rhodamine labelled tubulin or mCherry-EB3 and ET-405/488/561/647 for imaging SNAP-Alexa647. For simultaneous imaging of green and red fluorescence, we used the triple-band TIRF polychroic ZT405/488/561rpc (Chroma) and the triple-band laser emission filter ZET405/488/561m (Chroma), mounted in the metal cube (Chroma, 91032) together with Optosplit III beamsplitter (Cairn Research Ltd, UK) equipped with a double emission filter cube configured with ET525/50m, ET630/75m and T585LPXR (Chroma). We used sequential acquisition for triple colour imaging experiments.
Atherton J., Jiang K., Stangier M.M., Luo Y., Hua S., Houben K., van Hooff J.J., Joseph A.P., Scarabelli G., Grant B.J., Roberts A.J., Topf M., Steinmetz M.O., Baldus M., Moores C.A, & Akhmanova A. (2017). A structural model for microtubule minus-end recognition and protection by CAMSAP proteins. Nature structural & molecular biology, 24(11), 931-943.
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3

Multimodal Microscopy Imaging of Pancreatic Cells

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Fixed and stained INS-1E cells, EndoC-βH1, dispersed human pancreatic islets and live GFP–ELKS-expressing pancreatic islets were imaged using TIRFM performed on an inverted research microscope Nikon Eclipse Ti-E (Nikon) with a perfect focus system (PFS) (Nikon), equipped with a Nikon CFI Apo TIRF 100×1.49 NA oil objective (Nikon), Evolve 512 EMCCD (Photometrics) or Prime BSI camera (Photometrics) or CoolSNAP HQ2 CCD camera (Roper Scientific) and controlled with the MetaMorph 7.7.11.0 software (Molecular Devices). Images were projected onto the chip of an Evolve 512 camera with an intermediate lens 2.5× (Nikon C mount adapter 2.5×) or onto a CoolSNAP HQ2 or a Prime BSI chip without the lens. In all cases the final magnification was equal to 0.065 μm/pixel. To keep cells at 37°C a stage top incubator INUBG2E-ZILCS (Tokai Hit) was used. For excitation, 491 nm 100 mW Stradus (Vortran), 561 nm 100 mW Jive (Cobolt) and 642 nm 110 mW Stradus (Vortran) lasers were used. The microscope was equipped with an ET-GFP 49002 filter set (Chroma) for imaging of proteins tagged with GFP, an ET-mCherry 49008 (Chroma) and an ET-405/488/561/647 filter set. For presentation, images were adjusted for brightness using ImageJ 1.50b.
Noordstra I., van den Berg C.M., Boot F.W., Katrukha E.A., Yu K.L., Tas R.P., Portegies S., Viergever B.J., de Graaff E., Hoogenraad C.C., de Koning E.J., Carlotti F., Kapitein L.C, & Akhmanova A. (2022). Organization and dynamics of the cortical complexes controlling insulin secretion in β-cells. Journal of Cell Science, 135(3), jcs259430.
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