After cell seeding, the inlet of the perfusion chamber was connected to a programmable syringe pump to generate a continuous flow of culture medium on the basal side of the scaffold at a volumetric flow rate of 100 μl h−1 using a programmable syringe pump (Braintree Scientific). The apical side of the epithelial cells was submerged in medium for 2 d to allow for cell proliferation. During this culture period, the entire experimental setup was kept in a humidified cell culture incubator maintained at 37 °C and 5% CO2. Once a confluent monolayer was formed, medium was gently aspirated from the open well to expose the epithelial cells to an ALI. The engineered ocular surface tissue was then maintained at an ALI for 14 d in a humidified cell culture incubator. During ALI culture, the cells were sustained by a steady flow of medium through the perfusion chamber at a rate of 100 μl h−1.
Programmable syringe pump
Manufactured by Braintree Scientific
The Programmable Syringe Pump is a precision instrument designed to accurately and consistently dispense or aspirate liquids. It features programmable flow rates and volumes, allowing for controlled and repeatable liquid handling.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using programmable syringe pump
1
Ocular Surface Tissue Engineering
2
Quantitative Shadowgraphy Assessment of MINT Device
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To assess the performance of the MINT device for CED, we developed a shadowgraphy setup and quantitatively measured the volumetric distributions in agarose brain phantoms. The agarose gel was prepared by dissolving 0.6 wt% of agarose (IBI Scientific, Dubuque, IA) into deionized water. The solution was heated and stirred until it became clear and then poured into a custom-made clear-acrylic box (15 × 15 × 15 cm). The solution was left to cool, gelate overnight at 4°C, and refrigerated until used. The experimental setup is depicted in Figure 2A and consisted of the clear-acrylic box and a 3D printed top frame designed to securely fit onto the box and rigidly attach to the MRI-compatible stereotactic system SmartFrame (MRI Interventions, Inc., Irvine CA) to guide and adjust the catheter trajectory in the x, y, and z directions. To stabilize the catheter and provide additional support against potential rotation and translation, we used custom, 3D printed reducing tubes and a lateral press-fit post. The three flow inlet ports (Figure 2B ) on MINT were connected to a programmable syringe pump (Braintree Scientific, Inc., Braintree, MA) via 36” I.V. extension polyethylene tubing (Medline Industries, Inc., Mundelein, IL). Additional components of the setup included a backlight and a side mirror for optimal contrast and accurate reconstruction of the volumetric distribution profiles.
3
Reconstituting Contrived Tears with Lipids
4
Fabrication of TENPO Membranes for Microfluidics
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