MDA-MB-231 and HCC1937 cells were seed in 96-well plates for each cell line (TNBC and non-TNBC, ~13 cell lines) at 4000 cells per well, and cell viability assay was conducted with CCK-8 kit after combinational treatment of CB1 antagonists with ferroptosis inducers (erastin, RSL3, ML210, FIN56, CIL56) for 72 h according to the manufacturer’s instructions (Dojindo Laboratories, Japan). For cell death rescue experiments, the effects of various cell death pathway inhibitors were calibrated for each cell line for doses and effects. Ferroptosis inhibitors (Ferrostatin-1, GSH, and Deferoxamine), and cell death inhibitors were used to inhibit apoptosis (zVAD-FMK), necrosis (Necrostatin-1), autophagy (3-Methyladenine) at indicated concentrations 1 h before the treatment with combination therapy.
Fin56
Manufactured by Dojindo Laboratories
Sourced in Japan
FIN56 is a laboratory instrument designed for the analysis of cellular ferroptosis. It provides quantitative measurements of ferroptosis-associated parameters, including lipid peroxidation and cell viability.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fin56
1
Combination Therapy for TNBC Viability
2
Ferroptosis Induction in Cancer Cells
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HGC-27 and MGC-803 cells were seeded in 96-well plates at 5000 cells/well and cultured at 37 °C and 5% CO2 for 24 h. The ferroptosis inducer FIN56 (Dojindo, Japan) was dissolved in dimethyl sulfoxide to generate working concentrations of 0, 1, 2, 5, 10, 15, and 20 μM; each concentration was evaluated in six wells. Cell viability was measured using a Cell Counting-8 Kit (CCK-8, Beyotime, China). A microplate reader was used to determine the OD at an absorbance of 450 nm. After calculating the half-maximal inhibitory concentration of each cell line, the inhibition curve was plotted using the ggplot2 R tool.
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