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2 protocols using magnesium chloride 6 hydrate

1

Diabetes Compounds Characterization Protocol

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Alloxan monohydrate 98%, glucose oxidase-peroxidase reagent, sucrose, starch powder, and magnesium chloride-6-hydrate [MgCl2, 6H2O] were acquired from Sigma-Aldrich, Steinheim, Germany, as for the α-glucosidase enzyme, and acarbose were imported from Sigma-Aldrich, China, furthermore, the α-amylase enzyme and phlorizin dehydrate were both purchased from Sigma-Aldrich, USA, and Pentobarbital was imported from France. Dimethyl-sulfoxide (DMSO), Sodium chloride (NaCl) and potassium chloride (KCl) were purchased from Sigma Aldrich, Riedel-de Haen, Denmark. Ether and glibenclamide have been both acquired from Casablanca and Oujda respectively in Morocco, as for the Calcium chloride dihydrate [CaCl2, 2H2O] was imported from Spain, Scharlau Chemie, s.a. The sodium bicarbonate [NaHCO3] was purchased from Puerto Rico, (Farco Chemical Supply), and finally Sodium phosphate monobasic dihydrate [NaH2PO4 · 2H2O] from Panreac Spain.
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2

Antioxidant and Antidiabetic Potential Assay

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The following reagents were purchased from Sig-maAldrich: The starch and the sucrose powders, the αglucosidase enzyme, the α-amylase enzyme, dinitrosalicylic acid, Acarbose, phlorizin dehydrate, D-glucose anhydrous, potassium chloride (KCl), magnesium chloride-6-hydrate (MgCl 2 ꞏ6H 2 O), sodium chloride (NaCl), folinciocalteu, gallic acid, ascorbic acid, quercetin, DPPH, aluminum chloride (AlCl 3 ), potassium ferricyanide [K 3 Fe (CN) 6 ], methanol, ethanol, chloroform, and ferric chloride (FeCl 3 ), Trichloroacetic acid (TCA). Calcium chloride dihydrate (CaCl 2 ꞏ2H 2 O) purchased from ScharlauChemie S.A., Spain. Sodium hydrogen carbonate (NaHCO 3 ) were purchased from Farco Chemical Supplies, (Puerto Rico). Sodium phosphate monobasic 2-hydrate NaH 2 PO 4 ꞏ2H 2 O purchased from Panreac, Spain. Alloxan mono-hydrate was purchased from ACROS Organics. Pentobarbital obtained from CEVA Santé Animale, France. Ether obtained from Somaprol, Casablanca, Morocco.
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