Apc cy7 conjugated anti cd62l

For analysis of surface markers, single-cell suspensions were stained with following fluorochrome-conjugated antibodies following established guidelines (61 (link)): FITC-conjugated anti-CD45.1 (Biolegend, 110705), PerCP-Cyanine5.5-conjugated anti-CD8 (Biolegend, 110733), Pacific Blue-conjugated anti-CD8 (Biolegend, 100725), PE-conjugated anti-CD69 (Biolegend, 104508), FITC-conjugated TIM3 (Genetex, GTX54055), APC-conjugated anti-LAG3 (Biolegend, 125210), APC-conjugated anti-PD-1 (Biolegend, 135210), APC-conjugated anti-IL-2 (Biolegend, 503809), APC-conjugated anti-CD25 (Biolegend, 101909), FITC-conjugated anti-IFN-γ (Biolegend, 505805), APC-conjugated anti-TNF-α (Biolegend, 506307), PE-conjugated anti-H-2 K(b)/SIINFEKL Tetramer (Helixgen, HG08T14028), BV421-conjugated KLRG1 (Biolegend, 138413), BV605-CD127 (Biolegend, 135025), and APC-Cy7-conjugated anti-CD62L (Biolegend, 104427), For intracellular staining, cells were first fixed with IC Fixation Buffer (eBioscience 00-8222-49) and permeabilized with Permeabilization Buffer (Invitrogen, 00-8333-56). Flow cytometry was performed using Accuri C6 or Sony ID7000 system and analyzed with FlowJo software.