Antibodies used in these experiments included the following: anti-RND2 (13844–1-AP, Proteintech, USA), anti-Rho7/Rnd2 (GXT56070, GeneTex, USA), anti-p-p38 (#4511, Cell Signaling Technology, USA), anti-p38 (#9212, Cell Signaling Technology, USA), anti-cleaved-caspase3 (ab32042, Abcam, UK), anti-caspase3 (NB100-56708SS, Novus, USA), anti-BAX (50599–2-Ig, Proteintech, USA), anti-GAPDH (#5174, Cell Signaling Technology), anti-P62 (M162–3, Medical Biological Laboratories, Japan), anti-Beclin1 (11306–1-AP, Proteintech, USA), anti-LC3B (GB11124, Servicebio, China), anti-DYKDDDK/Flag-tag (ANT102, Antgene, China), and anti-His-tag (D291–3, Medical Biological Laboratories, Japan). The autophagy inhibitor wortmannin (3-MA) and the autophagy activator rapamycin (Sirolimus) (S1039, USA) were purchased from Selleck (S2758, USA).
Anti bax 50599 2 ig
Manufactured by Proteintech
Sourced in United States
Anti-Bax (50599-2-Ig) is a primary antibody product manufactured by Proteintech. It is designed to detect the Bax protein, which is a pro-apoptotic member of the Bcl-2 family. This antibody can be used in various laboratory techniques, such as Western blotting, immunohistochemistry, and immunofluorescence, to study the expression and localization of the Bax protein.
Automatically generated - may contain errors
Lab products found in correlation
Ab32042, by Abcam (2 mentions)
Anti p p38, by Cell Signaling Technology (2 mentions)
Nb100 56708ss, by Novus Biologicals (2 mentions)
Anti dykdddk flag tag ant102, by Antgene (2 mentions)
Anti beclin1 11306 1 ap, by Proteintech (2 mentions)
Anti p38, by Cell Signaling Technology (2 mentions)
Anti gapdh, by Cell Signaling Technology (2 mentions)
Sirolimus, by Selleck Chemicals (1 mentions)
Anti nox4, by Abcam (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti bcl2 26593 1 ap, by Proteintech (1 mentions)
Anti nf κb p65 sc 8008, by Santa Cruz Biotechnology (1 mentions)
Anti opa1, by Novus Biologicals (1 mentions)
Anti p nf κb p65, by Cell Signaling Technology (1 mentions)
Ab109268, by Abcam (1 mentions)
Anti mmp2 10 373 2 ap, by Proteintech (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Sc79 s7863, by Selleck Chemicals (1 mentions)
Anti cyclin d1 60186 1 ig, by Proteintech (1 mentions)
Quickblock blocking buffer, by Beyotime (1 mentions)
6 protocols using anti bax 50599 2 ig
1
Antibody Screening for Cell Signaling
2
LPS-Induced Inflammatory Cytokine Response
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LPS (L2630, E. coli 0111: B4) was purchased from Sigma-Aldrich (St. Louis, MO, United States). MaR1 was obtained from Cayman Chemical (Ann Arbor, MI, United States). The primary antibodies used in this study were as follows: anti-NOX4 (ab133303, Abcam), anti-TNF-α (AF7014; Affinity), anti-IL-6 (347023; Zenbio), anti-MCP-1 (AF-479-SP, R&D), anti-BAX (50599-2-Ig; Proteintech), anti-BCL-2 (26593-1-AP; Proteintech), anti-cleaved caspase-3 (9661; Cell Signaling Technology), anti-DRP-1 (NB110-55288; Novus), anti-MFN-1 (13798-1-AP; Proteintech), anti-OPA-1 (NB110-55290; Novus), anti-p-IκBα (AF2002; Affinity), anti-IκBα (AF5002; Affinity), anti-p-NF-κB P65 (3033; Cell Signaling Technology), anti-NF-κB P65 (sc-8008; Santa Cruz), and anti-GAPDH (ab8245, Abcam).
3
Phosphorylation Pathway Modulation Protocol
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GNE-477 [S6516, purity (98.43%)] (Figure 1A ) and the AKT phosphorylation activator SC79 (S7863, purity (>97%)) were both purchased from Selleckchem (Houston, TX, United States), and GNE-477 was dissolved and preserved in dimethyl sulfoxide (DMSO) obtained from Servicebio (G5051, Wuhan, China). The following antibodies were used: anti-AKT (product no.4691, Cell Signaling Technology (CST), United States), anti-phospho-AKT (product no. 4060; CST, United States), anti-CyclinD1 (60,186-1- Ig, Proteintech, Wuhan, China), anti-MMP2 (10,373-2- AP, Proteintech, Wuhan, China), anti-Bax (50599-2-Ig, Proteintech, Wuhan, China), anti-Bad (product no. 9239,CST,United States), anti-Bcl-2 (127891-AP,Proteintech, Wuhan, China), anti-mTOR (20657-1-AP, Proteintech, Wuhan, China), anti-phospho-mTOR (ab109268, Abcam, United Kingdom), and anti GAPDH (60004-1-Ig, Proteintech).
4
Quantifying ALKBH5, SOX9, Caspase3, Bax in iSCs
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The iSCs were harvested and extracted in strong RIPA lysis buffer (Beyotime, Shanghai, China) with 1% PMSF on ice and then denatured for 10 min at 99 °C supplemented with 5×SDS loading buffer. The total protein was separated by a 10% SDS-PAGE (Epizyme, Shanghai, China) and transferred to a PVDF membrane (Beyotime, Shanghai, China). After blocking the membrane with QuickBlock Blocking Buffer (Beyotime, Shanghai, China) at room temperature for 30 min, the membrane was incubated with antibodies at 4 °C overnight. Western blotting (WB) was performed using anti-ALKBH5 (ab195377; Abcam, Cambridge, UK, 1:500), anti-SOX9 (67439-1-Ig; Proteintech, Wuhan, China, 1:3000), anti-Caspase3 (14220; CST, Danvers, MA, USA, 1:1000), anti-Bax (50599-2-Ig; Proteintech Group, Wuhan, China, 1:2000), and anti-β-actin (60004-1-Ig; Proteintech, Wuhan, China, 1:10,000) antibodies. Finally, the secondary antibodies (anti-rabbit A0208; anti-mouse A0216; Beyotime, Shanghai, China, 1:1000) were incubated for another 1 h, and then chemiluminescent detection was performed.
5
Mitochondrial protein expression analysis
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Anti-COXIV (ab33985, RRID: AB_879754), VDAC1 (ab14734, RRID: AB_443084) and PGC-1α (ab191838, RRID: AB_2721267) antibodies were obtained from Abcam (Cambridge Science Park, Cambridge, UK). Anti-Caspase3 (9662, RRID: AB_331439) and cleaved-capase3 (9664, RRID: AB_2070042) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-Bax (50599-2-Ig, RRID: AB_2061561) , Cyt-c (10993-1-AP, RRID:AB_2090467) , NRF1 (12482-1-AP, RRID: AB_2282876), GAPDH (60004-1-Ig, RRID: AB_2107436), and β-tubulin (10094-1-AP, RRID: AB_2210695) antibodies were obtained from Proteintech (Rosemont, IL, USA). The anti-DsbA-L antibody was provided by Dr. Feng Liu(14). All secondary antibodies (MitoTracker Green FM and MitoTracker Red CMXRos) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). The calcium ionophore was purchased from Sigma-Aldrich (Shanghai, China). Antimycin A (>95% pure, ab141904) and a Mitochondria/Cytosol Fractionation Kit (ab65320) were purchased from Abcam (Cambridge Science Park, Cambridge, UK). The target sequence for mouse DsbA-L has been described previously.22 (link)
6
Antibodies for Autophagy Pathway Study
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Antibodies used in this experiment included the following items: anti-RND2 (13844-1-AP, Proteintech, USA), anti-Rho7 /Rnd2 (GXT56070, from GeneTex in USA), anti-p-p38(#4511, from Cell Signaling Technology in USA), anti-p38 (#9212, from Cell Signaling Technology in USA), anti-cleaved-caspase3 (ab32042, from Abcam in UK), anti-caspase3 (NB100-56708SS, from Novus in USA), anti-Bax (50599-2-Ig, from Proteintech in USA), anti-GAPDH (#5174, from Cell Signaling Technology), anti-SQSTM1/P62 (M162-3, from Medical Biological Laboratories in Japan), anti-Beclin1 (11306-1-AP, from Proteintech in USA), anti-LC3B (GB11124, from Servicebio in China), USA), anti-DYKDDDK/Flag-tag (ANT102, from Antgene in China), and anti-His-tag (D291-3, from Medical Biological Laboratories in Japan). Autophagy inhibitor Wortmannin (3-MA) and the autophagy activator Rapamycin (Sirolimus) (S1039, from USA) were bought from Selleck (S2758, USA).
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