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2 protocols using casp3 rabbit pab

1

Antibody Panel for Neural Lineage Analysis

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The following polyclonal (pAb) and monoclonal (mAb) primary antibodies used in the study were obtained from the indicated commercial sources: BAF155 rabbit pAb (1:20; Santa Cruz), BAF155 mouse mAb (1:100; Santa Cruz), BAF170 rabbit pAb (1:100; Bethyl), BAF170 rabbit pAb (1:100; Sigma), Olig2 rabbit pAb (1:200; Millipore), PDGFRα rat pAb (1:200; BD Bioscience), Sox9 rabbit pAb (1:100; Millipore), Sox10 Guinea pig pAb (1:100; a gift from Prof. Michael Wegner), Ki67 rabbit pAb (1:50; Novocastra), Ki67 mouse mAb (1:100; Novocastra), IdU mouse mAb (1:50; Becton Dickinson), Casp3 rabbit pAb (1:100; Cell Signaling).
Secondary antibodies used were horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1:10,000; Covance), HRP-conjugated goat anti-mouse IgG (1:5,000; Covance), HRP-conjugated goat anti-rat IgG (1:10,000; Covance), and Alexa 488-, Alexa 568-, Alexa 594- and Alexa 647-conjugated IgG (various species, 1:400; Molecular Probes).
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2

Multiplex Antibody Staining Protocol

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Polyclonal (pAb) and monoclonal (mAb) antibodies used in this study (working dilution; sources): BAF170 rabbit pAb (1:100; cat. IHC-00213 for IHC, Bethyl), Cre rabbit pAb (1:200, Millipore), Cre mouse mAb (1:100, Sigma), Tbr2 rabbit pAb (1:300; Chemicon), BrdU mouse mAb (1:40; CalTag), BrdU rat pAb (1:100; Abcam), Casp-3 rabbit pAb (1:100; Cell Signaling), Brm mouse mAb (1:50; BD Biosciences), Brg1 mouse mAb (1:50; Santa Cruz), GFAP chick pAb (1:200; Abcam), GFAP mouse mAb (1:100; Chemicon), Sox2 rabbit pAb (1:100; Chemicon), Sox2 mouse mAb (1:50; R&D Systems), Sox2 goat pAb (1:200; Santa Cruz), S100β rabbit pAb (200; Dako), Mcm2 mouse mAb (1:100; BD Biosciences), Dcx guinea pig pAb (1:200; Chemicon), NeuN mouse mAb (1:100; Chemicon), Cre mouse mAb (1:100; Sigma), Nestin mouse mAb (1:100; Chemicon), Ki67 rabbit pAb (1:200; Vector Laboratories), GS mouse mAb (1:200; Chemicon), TLX [20 (link)], Alexa 488, Alexa 568, Alexa 594, Alexa 647 (1:400; Molecular Probes).
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