For cytofluorimetric analysis 5 × 105 cells were incubated with conjugated monoclonal anti-CD34, anti-CD45, anti-CD73, anti-CD90 and anti-CD105 (from MACS Miltenyi Biotec). Cells were analysed on a FACSCanto flow cytometer (BD Biosciences). 7-AAD was used to exclude non-viable cells from the analysis. Data were analysed using FACSDiva Software (BD Biosciences).
Anti cd73
Manufactured by Miltenyi Biotec
Sourced in Germany
Anti-CD73 is a laboratory reagent used in flow cytometry applications. It is a monoclonal antibody that specifically binds to the CD73 cell surface protein, which is expressed on various cell types. The core function of Anti-CD73 is to facilitate the detection and analysis of CD73-expressing cells.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd105, by Miltenyi Biotec (2 mentions)
Anti cd45, by Miltenyi Biotec (2 mentions)
Anti cd34, by Miltenyi Biotec (1 mentions)
Anti cd90, by Miltenyi Biotec (1 mentions)
Facsdiva software, by BD (1 mentions)
Facscanto flow cytometer, by BD (1 mentions)
Anti cd4, by Miltenyi Biotec (1 mentions)
Anti cd11b m1 70, by BioLegend (1 mentions)
Anti icos c398.4a, by BioLegend (1 mentions)
Anti cd19 1d3, by BD (1 mentions)
Anti nk1, by Miltenyi Biotec (1 mentions)
Anti cd8a, by Miltenyi Biotec (1 mentions)
Lsrfortessa x 20, by BD (1 mentions)
Foxp3 transcription factor staining buffer set, by BD (1 mentions)
Anti cd29, by BD (1 mentions)
Anti cd90, by BD (1 mentions)
3 protocols using anti cd73
1
Flow Cytometry Analysis of MSCs
2
Multiparameter Flow Cytometry for Immune Profiling
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Purified anti-mouse CD16/32 (clone 93, BioLegend) was used for Fc receptor blocking.
For in vitro cell IR experiments, anti-CD73 (REA778, Miltenyi Biotec) was used at the appropriate dilutions.
For tumor-infiltrating immune cell staining, anti-CD45 (REA737), anti-CD4 (REA604), anti-CD8a (REA983), anti-NK1.1 (REA1162), anti-FoxP3 (REA788), anti-CD73 (REA778, Miltenyi Biotec), anti-CD19 (1D3, BD), anti-CD11b (M1/70) and anti-iCOS (C398.4A, BioLegend) antibodies were used. For membrane staining, cells were incubated with the antibody panel at the adapted concentrations for 20 min at 4°C. For FoxP3 staining, the Foxp3/Transcription Factor Staining Buffer Set (BD Biosciences) was used according to the manufacturer’s instructions. Samples were washed in FACS buffer and resuspended in 200 µL FACS buffer before acquisition. Samples were acquired on an LSR Fortessa X20 (BD, Franklin Lakes, New Jersey) with FACSDiva software, and data were analyzed with FlowJo V.10.0.7 software (Tree Star, Ashland, Oregon, USA).
For in vitro cell IR experiments, anti-CD73 (REA778, Miltenyi Biotec) was used at the appropriate dilutions.
For tumor-infiltrating immune cell staining, anti-CD45 (REA737), anti-CD4 (REA604), anti-CD8a (REA983), anti-NK1.1 (REA1162), anti-FoxP3 (REA788), anti-CD73 (REA778, Miltenyi Biotec), anti-CD19 (1D3, BD), anti-CD11b (M1/70) and anti-iCOS (C398.4A, BioLegend) antibodies were used. For membrane staining, cells were incubated with the antibody panel at the adapted concentrations for 20 min at 4°C. For FoxP3 staining, the Foxp3/Transcription Factor Staining Buffer Set (BD Biosciences) was used according to the manufacturer’s instructions. Samples were washed in FACS buffer and resuspended in 200 µL FACS buffer before acquisition. Samples were acquired on an LSR Fortessa X20 (BD, Franklin Lakes, New Jersey) with FACSDiva software, and data were analyzed with FlowJo V.10.0.7 software (Tree Star, Ashland, Oregon, USA).
3
Multiparametric Immunophenotyping of Stem Cells
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For each time point cells were harvested, washed with PBS supplemented with 1 % FBS and stained for 30 min at 4 °C with the following antibodies (1:10 in PBS, BD Pharmingen, USA): anti-CD90, anti-CD29, anti-CD166 (all conjugated with phycoerythrin PE); anti-CD105, anti-CD34, anti-CD45, anti-CD107, anti-HLA-DR, anti-CD14, anti-CD44 (all conjugated with fluorescein isothiocyanate FITC); anti-CD73 (conjugated with allophycocyanin APC, MiltenyiBiotec, Germany). Cells were then suspended in cold PBS, at least 30,000 events per sample were collected with CyAN ADP flow cytometer and subsequent histograms of number of events (Counts) vs. Fluorescence Log (FL) were obtained.
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