Total protein was prepared, and western blot analysis were performed as previously described [19] . We used the following primary antibodies: anti-CD81 (Abcam, cat# ab79559), anti-TSG101 (Abcam, cat# ab125011), anti-E-cadherin (Cell Signaling Technology, Danvers, MA, USA, Cat# 14472S), anti-Vimentin (Abcam, Cat# ab92547), anti-EGFR (Cell Signaling Technology, Cat# 4267S), anti-ERK antibody (Cell Signaling Technology, Cat# 4696S) and anti-p-ERK antibody (Cell Signaling Technology, Cat# 4370T), anti-androgen receptor (Abcam, Cat# ab74272), anti-β-actin (Cell Signaling Technology, Cat# 3700S), anti-GAPDH (Cell Signaling Technology, Cat# 5174S).
Anti androgen receptor
Manufactured by Abcam
Sourced in United States
The Anti-androgen Receptor antibody is a primary antibody that specifically binds to the androgen receptor protein. The androgen receptor is a nuclear receptor that mediates the physiological effects of androgens. This antibody can be used to detect and study the androgen receptor in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti erk antibody, by Cell Signaling Technology (3 mentions)
Anti e cadherin, by Cell Signaling Technology (3 mentions)
Anti vimentin, by Abcam (3 mentions)
Anti egfr, by Cell Signaling Technology (3 mentions)
Anti gapdh, by Cell Signaling Technology (3 mentions)
Anti β actin, by Cell Signaling Technology (3 mentions)
Anti p erk antibody, by Cell Signaling Technology (3 mentions)
Anti tsg101, by Abcam (3 mentions)
Anti cd81, by Abcam (3 mentions)
Pvdf membrane, by Merck Group (2 mentions)
Anti actin, by Merck Group (2 mentions)
Goat anti mouse hrp, by Merck Group (2 mentions)
Goat anti rabbit hrp, by Cell Signaling Technology (2 mentions)
Crescendo ecl substrate, by Merck Group (2 mentions)
Anti mcm4, by Cell Signaling Technology (2 mentions)
Anti smad2 3, by Cell Signaling Technology (2 mentions)
Anti mcm2, by Cell Signaling Technology (2 mentions)
Anti p21, by Santa Cruz Biotechnology (2 mentions)
Anti mcm2, by Abcam (2 mentions)
Anti n cadherin, by Cell Signaling Technology (2 mentions)
5 protocols using anti androgen receptor
1
Western Blot Analysis of Cell Markers
2
Placental and Embryonic Protein Isolation and Immunoblotting
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Protein was isolated from E13.5 placentas and embryos by acetone
precipitation from RNA-isolation buffer (Buffer RLT or TRK) and resuspending in
SUTEB loading buffer (8M Urea, 1% SDS, 10mM EDTA, 10mM Tris-HCl, pH 6.8).
Protein lysates were run on 4–20% SDS-PAGE acrylamide gels and
transferred to PVDF membrane (Millipore). Immunoblots were probed with anti-Mcm2
(Epitomics/Abcam), anti-Mcm2(Cell Signaling Technology), anti-androgen receptor
(Epitomics/Abcam), anti-SMAD2/3(Cell Signaling), anti-p21(Santa Cruz),
anti-MCM4(Cell Signaling Technology), anti-actin (Sigma). Secondary antibodies
used included goat anti-rabbit-HRP (Cell Signaling) and goat anti-mouse-HRP
(Sigma). Crescendo ECL substrate(Millipore) was used and immunoblots digitally
scanned using a cDigit scanner. Quantification of immunoblots was performed
using ImageStudio software.
precipitation from RNA-isolation buffer (Buffer RLT or TRK) and resuspending in
SUTEB loading buffer (8M Urea, 1% SDS, 10mM EDTA, 10mM Tris-HCl, pH 6.8).
Protein lysates were run on 4–20% SDS-PAGE acrylamide gels and
transferred to PVDF membrane (Millipore). Immunoblots were probed with anti-Mcm2
(Epitomics/Abcam), anti-Mcm2(Cell Signaling Technology), anti-androgen receptor
(Epitomics/Abcam), anti-SMAD2/3(Cell Signaling), anti-p21(Santa Cruz),
anti-MCM4(Cell Signaling Technology), anti-actin (Sigma). Secondary antibodies
used included goat anti-rabbit-HRP (Cell Signaling) and goat anti-mouse-HRP
(Sigma). Crescendo ECL substrate(Millipore) was used and immunoblots digitally
scanned using a cDigit scanner. Quantification of immunoblots was performed
using ImageStudio software.
3
Placental and Embryonic Protein Isolation and Immunoblotting
4
Western Blot Analysis of Cellular Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total proteins were prepared, and western blot analysis was performed as previously described [19] . We used the following primary antibodies: anti-CD81 (Abcam, Cat# ab79559), anti-TSG101 (Abcam, Cat# ab125011), anti-E-cadherin (Cell Signaling Technology, Danvers, MA, USA, Cat# 14472S), anti-Vimentin (Abcam, Cat# ab92547), anti-N-cadherin (Cell Signaling Technology, Cat# 13116S), anti-MMP-2 (Cell Signaling Technology, Cat# 40994S), anti-MMP-9 (Cell Signaling Technology, Cat# 13667T), anti-ZEB1 (Cell Signaling Technology, Cat# 70512S), anti-Snail (Cell Signaling Technology, Cat# 3879S), anti-Slug (Cell Signaling Technology, Cat# 9585S) anti-Twist1 (Cell Signaling Technology, Cat# 46702S), anti-EGFR (Cell Signaling Technology, Cat# 4267S), anti-ERK antibody (Cell Signaling Technology, Cat# 4696S) and anti-p-ERK antibody (Cell Signaling Technology, Cat# 4370T), anti-androgen receptor (Abcam, Cat# ab74272), anti-β-actin (Cell Signaling Technology, Cat# 3700S) and anti-GAPDH (Cell Signaling Technology, Cat# 5174S).
5
Comprehensive Western Blot Analysis of Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total proteins were prepared, and western blot analysis was performed as previously described [19 (link)]. We used the following primary antibodies: anti-CD81 (Abcam, Cat# ab79559), anti-TSG101 (Abcam, Cat# ab125011), anti-E-cadherin (Cell Signaling Technology, Danvers, MA, USA, Cat# 14472S), anti-Vimentin (Abcam, Cat# ab92547), anti-N-cadherin (Cell Signaling Technology, Cat# 13116S), anti-MMP-2 (Cell Signaling Technology, Cat# 40994S), anti-MMP-9 (Cell Signaling Technology, Cat# 13667T), anti-ZEB1 (Cell Signaling Technology, Cat# 70512S), anti-Snail (Cell Signaling Technology, Cat# 3879S), anti-Slug (Cell Signaling Technology, Cat# 9585S) anti-Twist1 (Cell Signaling Technology, Cat# 46702S), anti-EGFR (Cell Signaling Technology, Cat# 4267S), anti-ERK antibody (Cell Signaling Technology, Cat# 4696S) and anti-p-ERK antibody (Cell Signaling Technology, Cat# 4370T), anti-androgen receptor (Abcam, Cat# ab74272), anti-β-actin (Cell Signaling Technology, Cat# 3700S) and anti-GAPDH (Cell Signaling Technology, Cat# 5174S).
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