Log In Sign up
The largest database of trusted experimental protocols

Pna cy3 telo c probes

Manufactured by Agilent Technologies
Visit Supplier

The PNA-cy3-Telo-C probes are a type of laboratory equipment designed for specific applications. They are used for the detection and analysis of telomeric sequences in biological samples. The probes contain a peptide nucleic acid (PNA) molecule labeled with a cyanine-3 (cy3) fluorescent dye, which targets the cytosine-rich (C-rich) strand of the telomeric repeat sequence. The core function of these probes is to provide a tool for researchers to investigate and study telomere-related processes and dynamics in various biological systems.

Automatically generated - may contain errors

Lab products found in correlation

Dapi mounting media, by Vector Laboratories (2 mentions) Primary anti 53bp1 antibody, by Santa Cruz Biotechnology (2 mentions) Alexa 488 labeled secondary antibody, by Thermo Fisher Scientific (2 mentions)

2 protocols using pna cy3 telo c probes

1

Immunofluorescence Analysis of DNA Damage Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary fibroblasts from healthy controls, CTC1 mutation positive patients and LCC patients were fixed for 5 minutes in 4% vol/vol formaldehyde in H2O and permeabilized in PBS with 1% BSA 0.1% Triton X100. Cells were incubated with primary anti-53BP1 antibody (SantaCruz) for 1 hour at room temperature, and then with Alexa 488 labeled secondary antibody (Life Technology). Samples were fixed for 5 minutes in 4% vol/vol paraformaldehyde and dehydrated in successive 5 minutes baths of 70% Ethanol, 95% ethanol and 100% ethanol. PNA-cy3-Telo-C probes (DAKO) were hybridized according to the supplier’s recommendations. Briefly, probes were incubated with the samples for 5 minutes at 80°C, and left in the dark at room temperature for 90 minutes. Samples were then washed twice in 70% formamide, 10 mM Tris-HCL, and PBS, and mounted with DAPI mounting media (Vectashield).
Jenkinson E.M., Rodero M.P., Kasher P.R., Uggenti C., Oojageer A., Goosey L.C., Rose Y., Kershaw C.J., Urquhart J.E., Williams S.G., Bhaskar S.S., O’Sullivan J., Baerlocher G.M., Haubitz M., Aubert G., Barañano K.W., Barnicoat A.J., Battini R., Berger A., Blair E.M., Brunstrom-Hernandez J.E., Buckard J.A., Cassiman D.M., Caumes R., Cordelli D.M., De Waele L.M., Fay A.J., Ferreira P., Fletcher N.A., Fryer A.E., Goel H., Hemingway C.A., Henneke M., Hughes I., Jefferson R.J., Kumar R., Lagae L., Landrieu P.G., Lourenço C.M., Malpas T.J., Mehta S.G., Metz I., Naidu S., Õunap K., Panzer A., Prabhakar P., Quaghebeur G., Schiffmann R., Sherr E.H., Sinnathuray K.R., Soh C., Stewart H.S., Stone J., Van Esch H., Van Mol C.E., Vanderver A., Wakeling E.L., Whitney A., Pavitt G.D., Griffiths-Jones S., Rice G.I., Revy P., van der Knaap M.S., Livingston J.H., O’Keefe R.T, & Crow Y.J. (2016). Mutations in SNORD118 cause the cerebral microangiopathy leukoencephalopathy with calcifications and cysts. Nature genetics, 48(10), 1185-1192.
+ Open protocol
+ Expand
2

Immunofluorescence Analysis of DNA Damage Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary fibroblasts from healthy controls, CTC1 mutation positive patients and LCC patients were fixed for 5 minutes in 4% vol/vol formaldehyde in H2O and permeabilized in PBS with 1% BSA 0.1% Triton X100. Cells were incubated with primary anti-53BP1 antibody (SantaCruz) for 1 hour at room temperature, and then with Alexa 488 labeled secondary antibody (Life Technology). Samples were fixed for 5 minutes in 4% vol/vol paraformaldehyde and dehydrated in successive 5 minutes baths of 70% Ethanol, 95% ethanol and 100% ethanol. PNA-cy3-Telo-C probes (DAKO) were hybridized according to the supplier’s recommendations. Briefly, probes were incubated with the samples for 5 minutes at 80°C, and left in the dark at room temperature for 90 minutes. Samples were then washed twice in 70% formamide, 10 mM Tris-HCL, and PBS, and mounted with DAPI mounting media (Vectashield).
Jenkinson E.M., Rodero M.P., Kasher P.R., Uggenti C., Oojageer A., Goosey L.C., Rose Y., Kershaw C.J., Urquhart J.E., Williams S.G., Bhaskar S.S., O’Sullivan J., Baerlocher G.M., Haubitz M., Aubert G., Barañano K.W., Barnicoat A.J., Battini R., Berger A., Blair E.M., Brunstrom-Hernandez J.E., Buckard J.A., Cassiman D.M., Caumes R., Cordelli D.M., De Waele L.M., Fay A.J., Ferreira P., Fletcher N.A., Fryer A.E., Goel H., Hemingway C.A., Henneke M., Hughes I., Jefferson R.J., Kumar R., Lagae L., Landrieu P.G., Lourenço C.M., Malpas T.J., Mehta S.G., Metz I., Naidu S., Õunap K., Panzer A., Prabhakar P., Quaghebeur G., Schiffmann R., Sherr E.H., Sinnathuray K.R., Soh C., Stewart H.S., Stone J., Van Esch H., Van Mol C.E., Vanderver A., Wakeling E.L., Whitney A., Pavitt G.D., Griffiths-Jones S., Rice G.I., Revy P., van der Knaap M.S., Livingston J.H., O’Keefe R.T, & Crow Y.J. (2016). Mutations in SNORD118 cause the cerebral microangiopathy leukoencephalopathy with calcifications and cysts. Nature genetics, 48(10), 1185-1192.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!