Spermatozoa were donated by three healthy men and frozen unperturbed in seminal fluid to which colloidal gold was added (to be used as fiducial markers). A Vitrobot climate-controlled plunge freezer (FEI Company Ltd., Eindhoven, The Netherlands) was used within 1–3 h post ejaculation. After freezing for electron microscopy, remaining cells were examined under the light microscope, where their motility ensured that viable spermatozoa had been frozen.
Vitrobot climate controlled plunge freezer
Manufactured by Thermo Fisher Scientific
The Vitrobot climate-controlled plunge freezer is a laboratory equipment designed for preparing samples for cryogenic electron microscopy. It rapidly freezes samples in a controlled environment, preserving their native structure for subsequent analysis.
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Lab products found in correlation
2 protocols using vitrobot climate controlled plunge freezer
1
Freezing Viable Spermatozoa with Gold Markers
2
Unperturbed Sperm Cryopreservation Protocol
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Seven different ejaculates from one healthy donor (defined as having produced offspring) were collected according to the WHO's guidelines for sperm collection (34 ) and frozen unperturbed. A Vitrobot climate controlled plunge freezer (FEI Company Ltd, Eindhoven, The Netherlands) was used within 1–3 hours post-ejaculation. After freezing, remaining cells were examined under the light microscope, where their motility ensured that viable cells had been frozen.
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