Transgenic mice (C57B6.CgTg SOD1-G93A-1Gur/J) were originally from the Jackson Laboratory and later supplied by Professor Honglin Feng, Harbin Medical University, China. Stable breeding was maintained by mating purchased heterozygous SOD1-G93A male mice with C57BL/6 J female mice. The mice were kept in a temperature-controlled (22–24℃) room with a 12 h light and 12 h dark cycle providing clean drinking water and granular mouse feed, four per cage with a male and female ratio of 1:3. All animal protocols were reviewed and approved by the Institutional Animal Care and Use Committee of Shanxi Medical University (DW2022031).
hSOD1G93A-positive and age-matched negative mice were euthanized. The elected mice were anesthetized and perfused transcardially with phosphate-buffered saline (PBS, PH = 7.4) for 10 min, then 4% paraformaldehyde (PFA) in 0.1% PBS for 1 h. The spinal cord was dissected, post-fixed with 4% PFA for 48 h and then paraffin-embedded. A series of spinal cord sections were made and sectioned at a thickness of 5 μm. Muscle tissue was quickly removed from mouse gastrocnemius, then placed in liquid nitrogen and stored in a refrigerator.
hSOD1G93A-positive and age-matched negative mice were euthanized. The elected mice were anesthetized and perfused transcardially with phosphate-buffered saline (PBS, PH = 7.4) for 10 min, then 4% paraformaldehyde (PFA) in 0.1% PBS for 1 h. The spinal cord was dissected, post-fixed with 4% PFA for 48 h and then paraffin-embedded. A series of spinal cord sections were made and sectioned at a thickness of 5 μm. Muscle tissue was quickly removed from mouse gastrocnemius, then placed in liquid nitrogen and stored in a refrigerator.