The wt and ob/ob mice were weighed, and peripheral blood was collected for measurements of blood glucose and glycated hemoglobin (HbA1c). Blood was collected in sterile EDTA-treated tubes (#367838, BD Vacutainer; BD, Franklin Lakes, NJ) and stored at 4 °C. Blood glucose levels were measured using an Accu-Chek Performa blood glucose meter (Roche Diabetes Care, Sydney, Australia). HbA1c was measured using a Mouse Hemoglobin A1c Kit (#80310; Crystal Chem, Elk Grove Village, IL) according to the manufacturer’s instructions. HbA1c levels were read on a POLARstar Omega Spectrophotometer (BMG LABTECH, Ortenberg, Germany). Samples were averaged with n=8 per biological group, and statistical significance (p<0.05) was measured using a Student t test.
Accu chek performa blood glucose meter
Manufactured by Roche Diabetes Care
Sourced in Switzerland
The Accu-Chek Performa blood glucose meter is a laboratory equipment product designed to measure blood glucose levels. It provides a simple and efficient way to monitor and manage diabetes. The device offers accurate and reliable results, allowing healthcare professionals to make informed decisions about patient care.
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3 protocols using accu chek performa blood glucose meter
1
Characterizing Glucose Metabolism in Mice
2
Glycemic Index Measurement in Mice
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Three days before euthanasia, the mice were fasted for 12 h for the GI in vivo test. After a 12 h fasting period, a chow diet and bread sample for all four groups were fed to the mice, and then the tail tip of the mice was cut off. Five blood samples in each group were collected at 0, 30, 60, 90, and 120 min. All collected blood samples were used to determine the glucose levels in the samples with an Accu-Chek Performa blood glucose meter (Roche Diabetes Care, Switzerland). The GI was determined using the area under the curve (AUC) calculation method (Chlup et al., 2008 (link)).
3
Blood Glucose Monitoring Protocol
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The finger-tip blood sample Accu-Chek Performa Blood Glucose meter (Roche Diabetes Care, Inc. Switzerland) was used for assessing blood glucose levels. The reference range was a random plasma glucose ≥ 200 mg/dl (at any time of the day regardless of last meal time). Nurses were assigned to do the needle prick using disposable needles, while students were assigned to record the reading [23 (link)].
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