Rhodamine b rd

The BBB permeability was analyzed 1 week after occlusion of a penetrating arteriole. Rhodamine B (RD; 0.2 ml of 1% in saline; Sigma) was administered intravenously immediately prior to two-photon imaging to visualize the vasculature. Fluorescent intravascular dyes were used to measure leakage from the vasculature (26 (link), 27 (link)). The total fluorescence intensity in the extravascular compartment was analyzed (28 (link)). Images (512 × 512 pixels) were acquired using a 0.95 numerical aperture and × 25 water immersion objective 0, 5, 15, 30, and 60 min after administration of fluorescent dextran, and XYZ stacks of images were obtained.

Gelatin type A obtained from porcine skin, chitosan (448877-medium molecular weight (190,000–310,000 Da) and deacetylation degree of 75–85%), N-isopropylacrylamide monomers (NIPAM), acrylic acid (AA), N,N′-methylenebisacrylamide (BIS), ammonium persulfate (APS), sodium dodecyl sulfate (SDS), Span 80, glutaraldehyde, Rhodamine B (Rd) and PBS tablets were purchased from Sigma-Aldrich (St. Louis, MI, USA). Synthetic silicate nanoplatelets (Laponite XLG) were obtained from Southern Clay Products, Inc. (Louisville, KY, USA). The rest of materials were provided by the following companies: sodium acetate (Bio Basic, Markham, ON, Canada), Heavy mineral oil and n-hexane (Fisher Scientific, Pittsburgh, PA, USA), ethanol (Commercial Alcohols, Toronto, ON, Canada), acetic acid and toluene (VWR, Radnor, PA, USA), and dialysis bags (Spectrum Lab, Rancho Dominguez, CA, USA).