Immature DCs and mature DCs were stained with a LIVE/DEAD Fixable Dead Cell Stain Kit (Life Technologies, Grand Island, NY, USA), then fixed with 2% PFA. After fixation, the cells and appropriate controls were prepared. All samples were stained with HLA-DR-PE/Cy7 (Biolegend, San Diego, CA, USA), CD83-AF647 (Biolegend, San Diego, CA, USA), and CD83-PE (Biolegend, San Diego, CA, USA). Cells were analyzed on a BD LSR II (BD Biosciences, San Jose, CA, USA). Flow data were analyzed using FlowJo v9.6.1 software.
Cd83 af647
Manufactured by BioLegend
Sourced in United States
CD83-AF647 is a fluorescently labeled antibody targeting the CD83 cell surface protein. CD83 is a member of the immunoglobulin superfamily and is expressed on mature dendritic cells. The AF647 fluorophore is conjugated to the antibody, allowing for detection and analysis by flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Hla dr pe cy7, by BioLegend (2 mentions)
Live dead fixable dead cell stain kit, by Thermo Fisher Scientific (1 mentions)
Cd83 pe, by BioLegend (1 mentions)
Lsr 2, by BD (1 mentions)
Cd14 fitc, by BioLegend (1 mentions)
Moflo xdp, by Beckman Coulter (1 mentions)
Cd14 microbead kit, by Miltenyi Biotec (1 mentions)
2 protocols using cd83 af647
1
Assessing Dendritic Cell Maturation and Viability
2
Isolating and Analyzing Dendritic Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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CD14+ cells presorted by CD14 MicroBead Kit (Miltenyi Biotec Inc., San Diego, CA, USA), immature DCs, and mature DCs were stained with CD14-FITC (Biolegend, San Diego, CA, USA), HLA-DR-PE/Cy7 (Biolegend, San Diego, CA, USA), CD83-AF647 (Biolegend, San Diego, CA, USA) and Live/Dead-7-AAD (eBioscience, San Diego, CA) and sorted on a MoFlo XDP (Beckman Coulter Inc., Brea, CA, USA). Cells were collected in 100% FBS and pelleted for DNA/RNA extraction.
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