The primary antibodies used in this study included rabbit anti-GFP N-terminal antibody produced in rabbit (Sigma-Aldrich, cat. no. G1544-100UL), Anti-V5 Epitope Tag Antibody (Merck, cat. no. AB3792), rabbit anti-HA antibody produced in rabbit (Merck, cat. no. H6908-100UL), rabbit anti-FLAG antibody produced in rabbit (Merck, cat. no. F7425), rabbit anti-p-LvDorsal (Genecreate), rabbit anti-LvDorsal (Genecreate), and mouse anti-actin clone C4 antibody (Merc, cat. no. MAB1501) and Monoclonal anti-β-Actin antibody produced in mouse (Merck, cat. no. A2228). The secondary antibodies used were anti-mouse IgG horseradish peroxidase (HRP-conjugate) (Promega, cat. no. W4021), anti-rabbit IgG HRP-conjugate (Promega, cat. no. W4011), anti-rabbit IgG (H+L) F (ab’)2 fragment Alexa Fluor 488 Conjugate (CST, cat. no. 4412S), and anti-mouse IgG (H+L), F (ab’)2 Fragment Alexa Fluor 594 Conjugate (CST, cat. no. 8890S).
Rabbit anti gfp n terminal antibody
Manufactured by Merck Group
Sourced in United States
The Rabbit anti-GFP N-terminal antibody is a primary antibody that recognizes the N-terminal region of the Green Fluorescent Protein (GFP). It is designed for use in various biochemical and cell biology applications that require the detection of GFP-tagged proteins.
Automatically generated - may contain errors
2 protocols using rabbit anti gfp n terminal antibody
1
Antibody Validation for GFP, Epitope Tags
2
Detecting Recombinant Protein by Western Blot
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Total protein was isolated, resolved and transferred to membranes by SDS-PAGE and protein gel blot analysis as described (Hou et al., 2020) (link). Membranes were blotted with rabbit anti-GFP, N-terminal antibody (Sigma-Aldrich, St Louis, MO, USA). Blotted proteins were detected by secondary antibody Goat Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase (Sigma-Aldrich, USA) and SIGMA FAST™ BCIP/NBT (5-Bromo-4-chloro-3-indolyl phosphate/Nitro blue tetrazolium) Tablets (Sigma-Aldrich, USA).
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