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Anti phospho eif2α ser51 d9g8

Manufactured by Cell Signaling Technology

The Anti-phospho-eIF2α (Ser51) (D9G8) is a primary antibody that specifically recognizes the phosphorylated form of the eukaryotic translation initiation factor 2 alpha (eIF2α) at serine 51. This antibody is useful for detecting and analyzing the phosphorylation status of eIF2α, which is a key regulator of protein synthesis in cells.

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2 protocols using anti phospho eif2α ser51 d9g8

1

Eukaryotic Translation Initiation Complex Analysis

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Anti-eIF4AI (#2490, Cell signaling) (1:1000), anti-phospho-eIF2α (Ser51) (D9G8 #3398, Cell Signaling) (1:1000), anti-4E-BP1 (#9452, Cell Signaling) (1:2000), anti-phospho-4EBP (Thr37/46) (236B4 #2855, Cell Signaling) (1:2000), anti-β-actin (ab20272, Abcam) (1:1000), anti-eIF4E (#9742, Cell Signaling) (1:1000), anti-eIF4G (#2498, Cell Signaling) (1:1000), and anti-SBP-tag (SB19-C4 sc-101595, Santa Cruz Biotechnology) (1:1000) were used as primary antibodies. Chemiluminescence was induced by Pierce ECL Western Blotting Substrate (Thermo Scientific) and images were acquired by FluorChem R imaging sysmtem (ProteinSimple).
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2

Eukaryotic Translation Initiation Complex Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-eIF4AI (#2490, Cell signaling) (1:1000), anti-phospho-eIF2α (Ser51) (D9G8 #3398, Cell Signaling) (1:1000), anti-4E-BP1 (#9452, Cell Signaling) (1:2000), anti-phospho-4EBP (Thr37/46) (236B4 #2855, Cell Signaling) (1:2000), anti-β-actin (ab20272, Abcam) (1:1000), anti-eIF4E (#9742, Cell Signaling) (1:1000), anti-eIF4G (#2498, Cell Signaling) (1:1000), and anti-SBP-tag (SB19-C4 sc-101595, Santa Cruz Biotechnology) (1:1000) were used as primary antibodies. Chemiluminescence was induced by Pierce ECL Western Blotting Substrate (Thermo Scientific) and images were acquired by FluorChem R imaging sysmtem (ProteinSimple).
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