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Quaternary pump lc 40d

Manufactured by Shimadzu
Sourced in Japan

The Quaternary Pump (LC-40D) is a high-performance liquid chromatography (HPLC) pump developed by Shimadzu. The core function of this pump is to accurately and precisely deliver four different solvents simultaneously to the HPLC system at user-defined flow rates and proportions.

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2 protocols using quaternary pump lc 40d

1

Quantification of Chlorogenic Acid in Salak Seed Extract

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Quantification of chlorogenic acid in salak seed extract was coinducted using a high-performance liquid chromatography (HPLC) method [27 (link)] on a Shimadzu (Shimadzu Corp., Tokyo, Japan) quaternary pump (LC-40D) equipped with a diode array detector (SPD-M40) set at 325 nm, a column oven (CT0-40C), and an autosampler (SIL-40C). Data were collected using Shimadzu LabSolutions software Version 5.87 SP1. A Luna® C18 column (4.6 × 250 mm i.d., 5 µm) with a C18 guard column (Phenomenex, Torrance, CA, USA) was utilized. The detailed HPLC conditions used were the same with a previous study [27 (link)]. A calibration curve was produced by diluting standard chlorogenic acid with purity > 98.0% (Wuhan ChemFaces Biochemical Co., Ltd., Wuhan, China) within the range of 0.00625–0.1 mg/mL. Each sample was measured in triplicate.
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2

Quantification of Chlorogenic Acid in Salak Seed Extract

Check if the same lab product or an alternative is used in the 5 most similar protocols
Quantification of chlorogenic acid in salak seed extract was coinducted using a high-performance liquid chromatography (HPLC) method [27 (link)] on a Shimadzu (Shimadzu Corp., Tokyo, Japan) quaternary pump (LC-40D) equipped with a diode array detector (SPD-M40) set at 325 nm, a column oven (CT0-40C), and an autosampler (SIL-40C). Data were collected using Shimadzu LabSolutions software Version 5.87 SP1. A Luna® C18 column (4.6 × 250 mm i.d., 5 µm) with a C18 guard column (Phenomenex, Torrance, CA, USA) was utilized. The detailed HPLC conditions used were the same with a previous study [27 (link)]. A calibration curve was produced by diluting standard chlorogenic acid with purity > 98.0% (Wuhan ChemFaces Biochemical Co., Ltd., Wuhan, China) within the range of 0.00625–0.1 mg/mL. Each sample was measured in triplicate.
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