Cambridge gel doc system and software

BC cells were lysed using lysis buffer (Hepes pH 7.4 20 mM, NaCl 150 mM, 10% Glycerol, 1% Triton X-100) with protease inhibitors cocktail Complete (Roche Applied Science, Penzberg, Germany) and sodium orthovanadate or Phostop (Roche). Proteins were resolved on SDS-polyacrylamide gel electrophoresis and blotted on nitrocellulose (Thermo Fisher Scientific) or PVDF (Merck Millipore, Darmstadt, Germany) membranes. Detection was performed with ECL Detection Reagent (GE Healthcare) according to manufacturer’s protocol. ECL signals were detected, recorded and measured by either the GS-800 Calibrated Imaging Densitometer and the Quantity One Software (BioRad, Hercules, CA) or the Li-Cor scanner and Image Studio software (LI-COR Biosciences Inc., Lincoln, NE, USA) or the Uvitec Cambridge gel doc system and software (Cambridge, UK).

Cells were lysed using lysis buffer (hepes pH 7.4 20 mM, NaCl 150 mM, 10% glycerol, 1% triton X-100) with protease inhibitors cocktail complete (Roche Applied Science, Penzberg, Germany) and sodium orthovanadate or Phostop (Roche). Proteins were resolved using SDS-polyacrylamide gel electrophoresis and blotted on PVDF (Merck Millipore) membranes. Detection was performed using ECL detection reagent (BioRad, Hercules, CA, USA) according to the manufacturer’s protocol. ECL signals were detected, recorded using the Uvitec Cambridge gel doc system and software (Cambridge, UK).