Monoclonal anti chicken egg albumin clone ova 14 mouse igg1 isotype

Muramyl dipeptide (MDP) was obtained from Invivogen, Inc., (San Diego, CA, USA). The lipidated NOD2 agonists (SG29, SG115, and ZSB63) were synthesized as described [8 (link),24 (link)]. The HPLC purity of all pharmacologically investigated compounds was >95%. Bovine serum albumin, Tween 20, monoclonal anti-chicken egg albumin (clone OVA-14 mouse IgG1 isotype), and o-phenylenediaminedihydrochloride were obtained from Sigma (St. Louis, MO, USA). Horseradish-peroxidase-conjugated goat anti-mouse IgG (HRP-anti-mouse IgG) was obtained from Bio-Rad Laboratories (Hercules, CA, USA). Monoclonal antibodies (biotin-conjugated rat anti-mouse IgG1 and anti-mouse IgG2a) and streptavidin-HRP conjugate were purchased from PharMingen, Becton Dickinson (Franklin Lakes, NJ, USA). Chemicals used for the preparation of solutions and buffers were purchased from Kemika (Zagreb, Croatia). Ovalbumin (OVA) was purchased from Serva (Heidelberg, Germany). Egg-phosphatidylcholine (L-α-Phosphatidylcholine, type XI-E) was purchased from Avanti Polar Lipids. Cholesterol from the porcine liver was purchased from Sigma (USA).

Bovine serum albumin, Tween 20, monoclonal
anti-chicken egg albumin
(clone OVA-14 mouse IgG1 isotype), o-phenylenediaminedihydrochloride,
and MDP were from Sigma (U.S.A.). Horseradish-peroxidase-conjugated
goat anti-mouse IgG (HRP-anti-mouse IgG) was from Bio-Rad Laboratories
(U.S.A.). Biotin-conjugated rat anti-mouse IgG1 and anti-mouse IgG2a
monoclonal antibodies and streptavidin–peroxidase were from
PharMingen, Becton Dickinson (U.S.A.). Chemicals for buffers and solutions
were from Kemika (Croatia). Ovalbumin was from Serva (Germany). l-α-Phosphatidylcholine, type XI-E, from fresh egg yolk
(egg-phosphatidylcholine) was from Avanti Polar Lipids. Cholesterol
from porcine liver and dicetylphosphate were from Sigma (U.S.A.).
Monomannosyl-PEG-palmitic acid derivative (Man-PEG-Pam) was synthesized
as previously described.^{129 (link)}