Anti pi 3 5 p2 z p035

Manufactured by Echelon Biosciences

Anti-PI(3,5)P2 (Z-P035) is a reagent used for the detection and quantification of phosphatidylinositol-3,5-bisphosphate [PI(3,5)P2], a lipid signaling molecule found in eukaryotic cells. This product can be used in various biochemical and cell-based assays to study the role and regulation of PI(3,5)P2 in cellular processes.

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Lab products found in correlation

Beclin 1, by Cell Signaling Technology (1 mentions) Alexa fluor 568, by Thermo Fisher Scientific (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Atg16l1, by Cell Signaling Technology (1 mentions) Anti lamp1 h4a3, by Developmental Studies Hybridoma Bank (1 mentions) Alexa coupled secondary antibody, by Thermo Fisher Scientific (1 mentions) Hrp coupled secondary antibody, by Cell Signaling Technology (1 mentions) Anti α sma, by Merck Group (1 mentions) Anti flag m2, by Merck Group (1 mentions) Anti col1, by Cell Signaling Technology (1 mentions) Anti smad2 3 sc 133098, by Santa Cruz Biotechnology (1 mentions) Anti α sma, by Cell Signaling Technology (1 mentions) Apilimod, by Axon Medchem (1 mentions)

Spelling variants of this product

PI(3,5)P2 (4 citations)

2 protocols using anti pi 3 5 p2 z p035

Autophagy Pathway Modulation Reagents

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SB203580, SB202190, SP600125, PD98059, LY294002, Bafilomycin A1, and rapamycin were obtained from Alexis Corp. (San Diego, CA) or Tocris Cookson (Ellisville, MO). All other chemicals were of analytical grade and purchased from Sigma-Aldrich or EMD Biosciences (Gibbstown, NJ). Antibodies to ATG5 (#2630), ATG12 (#4180), ATG16L1 (#8089), Beclin 1 (#3738), β-actin (#4970), CTSD (#2284), LC3B (#2775), p62 (#5114), p38α (#9217), p38β (#2339), phospho-p38α (pThr-180/pThr-182; #9216), phospho-hsp27 (pSer-82; #2406), phospho-MK2 (pThr-222; #3316), Rab7 (#95746), and Rab9 (#5118) were purchased from Cell Signaling Technology (Danvers, MA). Recombinant GST-PIKfyve (full-length, #11-118) was obtained from Carna Biosciences (Natick, MA); and anti-PI(3,5)P2 (Z-P035) was obtained from Echelon Biosciences (Salt Lake City, UT). Anti-tubulin (RE11250C100) was obtained from BioVendor (Candler, NC). Anti-LAMP1 (H4A3) was purchased from Developmental Studies Hybridoma Bank at the University of Iowa (Iowa City, IA). Alexa Fluor™ 488, Alexa Fluor™ 568, and HRP-conjugated secondary antibodies to rabbit and mouse IgG were obtained from Molecular Probes^® Invitrogen (Carlsbad, CA), DakoCytomation, Sigma-Aldrich (St. Louis, MO), and Thermo Fisher Scientific (Waltham, MA).

Wible D.J., Parikh Z., Cho E.J., Chen M.D., Jeter C.R., Mukhopadhyay S., Dalby K.N., Varadarajan S, & Bratton S.B. (2024). Unexpected inhibition of the lipid kinase PIKfyve reveals an epistatic role for p38 MAPKs in endolysosomal fission and volume control. Cell Death & Disease, 15(1), 80.

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Antibody Reagents for Cellular Studies

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Antibodies used in this study are: anti-Hsp90 (sc-13119), anti-GAPDH (sc-32233) and anti-Smad2/3 (sc-133098) from Santa Cruz Biotechnology; anti-αSMA (A5228, for western-blot) and anti-flag M2 from Sigma; anti-CD68 (GeneTex GTX41864), anti-GM130 (#610822) from BD Biosciences; anti-EEA1 (GTX109638) and anti-caveolin-1 (GTX100205) from GeneTex; anti-CD63 (MCA2142) was from AbD Serotec; anti-EGFR (D38B1), anti-phospho-Smad2 (#3108) and 3 (#9520), anti-αSMA (#19245, for immunofluorescence), anti-COL1 (#91144) from Cell Signaling Technologies; anti-PI(3,5)P₂ (Z-P035) from Echelon; anti-HA.11 (16B12, Covance). Fluorescent Alexa-coupled secondary antibodies and DAPI were from Life Technologies and HRP-coupled secondary antibodies from Cell Signaling Technology. Apilimod was purchased from Axon Medchem. The plasmids encoding GFP-ICAM1 and P2Y12-GFP were as described 18 (link). All other chemicals were from Sigma unless otherwise stated.

Cinato M., Guitou L., Saidi A., Timotin A., Sperazza E., Duparc T., Zolov S.N., Giridharan S.S., Weisman L.S., Martinez L.O., Roncalli J., Kunduzova O., Tronchere H, & Boal F. (2021). Apilimod alters TGFβ signaling pathway and prevents cardiac fibrotic remodeling. Theranostics, 11(13), 6491-6506.

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