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Diphenylene iodonium

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Diphenylene iodonium is a chemical compound that functions as an inhibitor of NADPH oxidases, a family of enzymes involved in the production of reactive oxygen species. It has applications in various areas of scientific research, including the study of cellular signaling and oxidative stress.

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Lab products found in correlation

Superoxide dismutase, by Merck Group (2 mentions) Jsh 23, by Santa Cruz Biotechnology (2 mentions) Nigericin, by Cayman Chemical (1 mentions) Bapta am, by Cayman Chemical (1 mentions) Arl 67156 trisodium salt, by Bio-Techne (1 mentions) Jte 013, by Bio-Techne (1 mentions) Tolfenamic acid, by Merck Group (1 mentions) Flufenamic acid, by Thermo Fisher Scientific (1 mentions) Dcpib, by Merck Group (1 mentions) 4 hydroxycoumarin, by Thermo Fisher Scientific (1 mentions) Sphingosine 1 phosphate (s1p), by Cayman Chemical (1 mentions) Adenosine triphosphate (atp), by Merck Group (1 mentions) Lipopolysaccharide lps, by Merck Group (1 mentions) Zafirlukast, by Tokyo Chemical Industry (1 mentions)

3 protocols using diphenylene iodonium

1

Myeloid cell superoxide detection

Cited 1 time
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O2•− producing MDRC were detected by flow cytometry after staining with myeloid cell-specific antibodies and incubation for 20 min at RT with dihydroxyethidium (DHE, 10 μM; Molecular Probes, Eugene, OR) as described before 32 (link). The specificity of DHE for O2•− was validated by inducing a respiratory burst in the sorted myeloid cells by incubation at 37°C for 15 min with phorbol myristate acetate (PMA, 1 μg/ml) or PMA + the NADPH oxidase inhibitor diphenylene iodonium (DPI, 1 μM; Tocris Bioscience, Bristol, UK) , in presence of superoxide dismutase (256mU/ml, Sigma, St. Louis, MO) or in presence or absence of 10μM Pyrrolidine dithiocarbamate (Calbiochem, La Jolla, CA) or in the presence or absence of 5 μM NF- κB activation inhibitor II, JSH-23 ( Santa Cruz Biotechnology, Santa Cruz, CA ).
Wang Y., Jin T.H., Farhana A., Freeman J., Estell K., Zmijewski J., Gaggar A., Thannickal V.J., Schwiebert L.M., Steyn A.J, & Deshane J.S. (2014). Exposure to cigarette smoke impacts myeloid-derived regulatory cell function and exacerbates airway hyper-responsiveness. Laboratory investigation; a journal of technical methods and pathology, 94(12), 1312-1325.
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2

Myeloid cell superoxide detection

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
O2•− producing MDRC were detected by flow cytometry after staining with myeloid cell-specific antibodies and incubation for 20 min at RT with dihydroxyethidium (DHE, 10 μM; Molecular Probes, Eugene, OR) as described before 32 (link). The specificity of DHE for O2•− was validated by inducing a respiratory burst in the sorted myeloid cells by incubation at 37°C for 15 min with phorbol myristate acetate (PMA, 1 μg/ml) or PMA + the NADPH oxidase inhibitor diphenylene iodonium (DPI, 1 μM; Tocris Bioscience, Bristol, UK) , in presence of superoxide dismutase (256mU/ml, Sigma, St. Louis, MO) or in presence or absence of 10μM Pyrrolidine dithiocarbamate (Calbiochem, La Jolla, CA) or in the presence or absence of 5 μM NF- κB activation inhibitor II, JSH-23 ( Santa Cruz Biotechnology, Santa Cruz, CA ).
Wang Y., Jin T.H., Farhana A., Freeman J., Estell K., Zmijewski J., Gaggar A., Thannickal V.J., Schwiebert L.M., Steyn A.J, & Deshane J.S. (2014). Exposure to cigarette smoke impacts myeloid-derived regulatory cell function and exacerbates airway hyper-responsiveness. Laboratory investigation; a journal of technical methods and pathology, 94(12), 1312-1325.
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3

Evaluating Modulation of Ion Channels

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Chemicals are commercially available: DCPIB (Sigma-Aldrich, #SML2692-25MG), ARL 67156 trisodium salt (Tocris, #1283), S1P (Cayman, #62570), W146 (Tocris, #36024), JTE013 (Tocris, #2392), BAPTA AM (Cayman, #15551), diphenyleneiodonium (Tocris, #0504), ATP (Sigma-Aldrich, #A2382-5G), lipopolysaccharide (LPS) (Sigma-Aldrich, #L2880-100MG), nigericin (Cayman, 11437), dicumarol (Acros Organics, #204120050), chenodiol (VWR 21947-5G), tioxolone (Sigma-Aldrich, #217077), tolfenamic acid (Sigma-Aldrich, #T0535-5G), flufenamic acid (Acros Organics, #165920100), zafirlukast (TCI America, #Z0029-100MG), 4-hydroxycoumarin (Acros Organics, #121100050), and warfarin (Agilent, #PST-1000). All chemicals were dissolved with the manufacturers’ instructions. Dicumarol stock solution (10 to 20 mM) was made in dimethyl sulfoxide with brief sonication (2 to 5 min) and heating (50°C).
Chu J., Yang J., Zhou Y., Chen J., Chen K.H., Zhang C., Cheng H.Y., Koylass N., Liu J.O., Guan Y, & Qiu Z. (2023). ATP-releasing SWELL1 channel in spinal microglia contributes to neuropathic pain. Science Advances, 9(13), eade9931.
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