Pge2 elisa kit mouse pge2

RAW 264.7 cells were plated at a density of 1.5 × 10⁵ cells/well in 24-well plates and incubated for 24 h. Cells were treated with varying concentrations of either FM or FMP (12.5, 25, 50, and 100 µM) (then with or without LPS (1 µg/mL)) for 24 h. Cell culture supernatant (100 µL) was mixed with Griess reagent (Sigma-Aldrich, St. Louis, MA, USA) (100 µL), and absorbance was determined at 540 nm to measure NO production. The concentration of PGE₂ in the culture supernatant of cells treated with FMP 12.5, 25, 50, and 100 µM was detected using a PGE₂ ELISA Kit (Mouse PGE₂, R&D Systems, MN, USA).

RAW 264.7 cells were plated at a density of 1.0 × 10⁵ cells/well in 24-well plates and incubated for 24 h. Cells were treated with varying concentrations of 7AC (50, 100 and 200 µM) with or without LPS (1 µg/mL) for 24 h. After incubation, cell culture supernatant (100 µL) was mixed with Griess reagent (100 µL; Sigma-Aldrich, St. Louis, MA, USA) and absorbance was determined at 540 nm to measure NO production. The PGE₂ concentration in the culture supernatant of 7AC (50, 100 and 200 µM)-treated cells was estimated using a PGE₂ ELISA Kit (Mouse PGE₂, R&D Systems, Minneapolis, MN, USA).