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Dionex rp hplc system ultimate 3000

Manufactured by Thermo Fisher Scientific
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The Dionex RP-HPLC system (Ultimate 3000) is a high-performance liquid chromatography (HPLC) system designed for reverse-phase chromatography applications. It features a modular design and advanced components to deliver reliable and consistent performance.

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Dionex Ultimate 3000 HPLC system Dionex Ultimate 3000 HPLC Ultimate 3000 RP-HPLC Dionex Ultimate 3000 system Dionex UltiMate HPLC system Dionex 3000 HPLC system Ultimate 3000 HPLC system Dionex Ultimate 3000 Ultimate 3000 HPLC Dionex HPLC system

2 protocols using dionex rp hplc system ultimate 3000

1

Peptide Purification and Analysis via RP-HPLC

Check if the same lab product or an alternative is used in the 5 most similar protocols
A Thermo Scientific Dionex RP-HPLC system (Ultimate 3000) was used to purify and analyse the peptides. Vydac C18 analytical (5 μm) and semi-preparative (5 μm) columns were used. Analytical runs were performed with a 10-70% B gradient over 30 min at 1 mL/min, while semi-preparative runs were optimised for each peptide at 4.7 mL/min. Aqueous CH3CN containing 0.1% TFA was used for buffer A (5%, v/v) and buffer B (95%, v/v). Detection was done at 230 and 214 nm.
Hammond K., Cipcigan F., Al Nahas K., Losasso V., Lewis H., Cama J., Martelli F., Simcock P.W., Fletcher M., Ravi J., Stansfeld P.J., Pagliara S., Hoogenboom B.W., Keyser U.F., Sansom M.S., Crain J, & Ryadnov M.G. (2021). Switching Cytolytic Nanopores into Antimicrobial Fractal Ruptures by a Single Side Chain Mutation. ACS nano, 15(6), 9679-9689.
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2

Peptide Purification and Analysis via RP-HPLC

Check if the same lab product or an alternative is used in the 5 most similar protocols
A Thermo Scientific Dionex RP-HPLC system (Ultimate 3000) was used to purify and analyse the peptides. Vydac C18 analytical (5 μm) and semi-preparative (5 μm) columns were used. Analytical runs were performed with a 10-70% B gradient over 30 min at 1 mL/min, while semi-preparative runs were optimised for each peptide at 4.7 mL/min. Aqueous CH3CN containing 0.1% TFA was used for buffer A (5%, v/v) and buffer B (95%, v/v). Detection was done at 230 and 214 nm.
Hammond K., Cipcigan F., Al Nahas K., Losasso V., Lewis H., Cama J., Martelli F., Simcock P.W., Fletcher M., Ravi J., Stansfeld P.J., Pagliara S., Hoogenboom B.W., Keyser U.F., Sansom M.S., Crain J, & Ryadnov M.G. (2021). Switching Cytolytic Nanopores into Antimicrobial Fractal Ruptures by a Single Side Chain Mutation. ACS nano, 15(6), 9679-9689.
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