Alexa fluor 488 anti rabbit igg antibody

Dopamine (DA), ascorbic acid (AA), cysteine (Cus), uric acid (UA), serotonin, glucose, dihydroxyphenylacetic acid (DOPAC), alanine (Ala); arginine (Arg); asparagine (Asn); aspartic acid (Asp); glycine (Gly); histidine (His); trans-4-hydroxyl-l-proline (Hyp); isoleucine (Ile); leucine (Leu); lysine (Lys) and phosphate-buffered saline solution (PBS) all purchased from Sigma–Aldrich. Alexa Fluor^® 488 anti-rabbit-IgG antibody, DMEM/F12 medium, rabbit anti-v-Glut antibody (Abcam, Melbourne, Australia), mouse anti-GAD67 (EMD Millipore, Bayswater, Australia) and goat anti-rabbit (555 nm) purchased from Molecular probes, Life Technology.

The sections of liver tissues were blocked with 3% BSA in PBS for 1 h at room temperature, and then incubated with primary antibodies overnight at 4°C. The primary antibodies were anti-Ly6C/Ly6G antibody (Bio X Cell, West Lebanon, NH), anti-F4/80 antibody (Abcam, Cambridge, MA, USA) and anti-fibronectin antibody (Abcam). After washing in PBS for three times, the sections were incubated with Alexa Fluor 555 anti-rat IgG antibody or Alexa Fluor 488 anti-rabbit IgG antibody (Abcam) at room temperature for 1 h in the dark. After counterstaining with DAPI (Beyotime), the sections were observed and photographed with a Leica TCS SP8 Confocal Microscope (Buffalo Grove, IL, USA).