Full-length H19 and its antisense RNA were transcribed in vitro using TranscriptAid T7 High Yield Transcription Kit (#K0441, Thermo), treated with RNase-free DNase I (Takara), and purified with GeneJET RNA Purification Kit (#K0731, Thermo) according to the manufacturer’s instruction. Then, RNAs were labeled with desthiobiotinylate using PierceTM RNA 3′end Desthiobiotinylation Kit (#20163, Thermo). RNA pull-down assay was performed using PierceTM Magnetic RNA-protein Pull Down Kit (#20164, Thermo) according to the manufacturer’s instruction. Finally, the RNA-binding proteins were analyzed by liquid chromatography-mass spectrometry (LC-MS) (Triple TOF 6600 LC-MS, AB SCIEX, USA) or followed by western blot.
Triple tof 6600 lc ms
Manufactured by AB Sciex
Sourced in United States
The Triple TOF 6600 LC-MS is a high-resolution, high-mass accuracy mass spectrometry system designed for advanced analytical applications. It combines a triple quadrupole and a time-of-flight (TOF) analyzer to provide accurate mass measurements and high-throughput data acquisition.
Automatically generated - may contain errors
Lab products found in correlation
Pierce magnetic rna protein pull down kit, by Thermo Fisher Scientific (2 mentions)
Transcriptaid t7 high yield transcription kit, by Thermo Fisher Scientific (2 mentions)
Pierce rna 3 end desthiobiotinylation kit, by Thermo Fisher Scientific (2 mentions)
Rnase free dnase 1, by Takara Bio (2 mentions)
Genejet rna purification kit, by Thermo Fisher Scientific (1 mentions)
2 protocols using triple tof 6600 lc ms
1
RNA-protein Interactome Profiling
2
Identification of H19 RNA-binding Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Full-length H19 and its antisense RNA were transcribed in vitro using TranscriptAid T7 High Yield Transcription Kit (#K0441, Thermo), treated with RNase-free DNase I (Takara), and puri ed with GeneJET RNA Puri cation Kit (#K0731, Thermo) according to the manufacturer's instruction. Then, RNAs were labeled with desthiobiotinylate using PierceTM RNA 3′end Desthiobiotinylation Kit (#20163, Thermo).
RNA pull-down assay was performed using PierceTM Magnetic RNA-protein Pull Down Kit (#20164, Thermo) according to the manufacturer's instruction. Finally, the RNA-binding proteins were analyzed by liquid chromatography-mass spectrometry (LC-MS) (Triple TOF 6600 LC-MS, AB SCIEX, USA) or followed by western blot.
RNA pull-down assay was performed using PierceTM Magnetic RNA-protein Pull Down Kit (#20164, Thermo) according to the manufacturer's instruction. Finally, the RNA-binding proteins were analyzed by liquid chromatography-mass spectrometry (LC-MS) (Triple TOF 6600 LC-MS, AB SCIEX, USA) or followed by western blot.
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