Brain tissues from rats with cerebral ischemia and sham‐operated rats were homogenized in TRIzol and then extracted with chloroform. A small amount of the aqueous phase (1.2 ml) after chloroform extraction and centrifugation was adjusted to 35% ethanol and added to the RNeasy column. RNA was eluted following the protocol of the RNeasy kit (Qiagen, Hilden, Germany). RNA integrity was checked by electrophoresis, and RNA concentration was determined by UV spectrophotometry. cDNA was synthesized by reverse transcription using SuperScript III reverse transcriptase (Invitrogen Inc., Carlsbad, CA, USA) and hybridized to Affymetrix Rat Genome 430 2.0 array (Affymetrix, Santa Clara, CA, USA). A 200‐μl mixture containing 15 μg cDNA was loaded onto the microarray, and the microarray was hybridized at 45°C for 16 h and then placed into Affymetrix GeneChip Scanner for scanning and analysis to obtain rat mRNA expression data.
Affymetrix rat genome 430 2.0 array
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Affymetrix Rat Genome 430 2.0 Array is a microarray platform designed for the comprehensive analysis of gene expression in rat samples. The array contains approximately 31,000 probe sets, representing over 30,000 transcripts and variants from the rat genome. It provides broad coverage of the rat transcriptome and enables researchers to study gene expression patterns in a wide range of biological samples and experimental conditions.
Automatically generated - may contain errors
3 protocols using affymetrix rat genome 430 2.0 array
1
Rat Brain Ischemia mRNA Profiling
2
Transcriptomic Analysis of Diabetic Nephropathy
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The kidney tissues of NC rats and DN rats were homogenized in TRIzol, and then extracted with chloroform. After chloroform extraction and centrifugation, a small amount of aqueous phase (1.2 mL) was adjusted to 35% ethanol and added to RNeasy column. The RNA was eluted according to the RNaeasy kit (Qiagen, CA), and the integrity of RNA was checked by electrophoresis, and the concentration was determined by an ultraviolet spectrophotometer. cDNA was synthesized by the Superscript ™ III reverse transcription (Invitrogen) and hybridized with Affymetrix Rat Genome 430 2.0 array (Affymetrix, CA). A 200 μL mixture containing 15 μg cRNA was loaded onto the chip. The chip was hybridized at 45°C
for 16 hours and then put into Affymetrix GeneChip scanner for scanning analysis. The differential expression genes were screened and a heatmap was plotted according to |FoldChange| > 2 and P < 0.01.
for 16 hours and then put into Affymetrix GeneChip scanner for scanning analysis. The differential expression genes were screened and a heatmap was plotted according to |FoldChange| > 2 and P < 0.01.
3
Transcriptomic Analysis of Diabetic Nephropathy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The kidney tissues of NC rats and DN rats were homogenized in TRIzol, and then extracted with chloroform. After chloroform extraction and centrifugation, a small amount of aqueous phase (1.2 mL) was adjusted to 35% ethanol and added to RNeasy column. The RNA was eluted according to the RNaeasy kit (Qiagen, CA), and the integrity of RNA was checked by electrophoresis, and the concentration was determined by an ultraviolet spectrophotometer. cDNA was synthesized by the Superscript ™ III reverse transcription (Invitrogen) and hybridized with Affymetrix Rat Genome 430 2.0 array (Affymetrix, CA). A 200 μL mixture containing 15 μg cRNA was loaded onto the chip. The chip was hybridized at 45°C
for 16 hours and then put into Affymetrix GeneChip scanner for scanning analysis. The differential expression genes were screened and a heatmap was plotted according to |FoldChange| > 2 and P < 0.01.
for 16 hours and then put into Affymetrix GeneChip scanner for scanning analysis. The differential expression genes were screened and a heatmap was plotted according to |FoldChange| > 2 and P < 0.01.
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