Qpcr assay kits

2-Chloroethanol (2-CE) was obtained from Sinopharm Chemical Reagent Co., Ltd. (Ningbo, China), while reagents for the cell culture were purchased from Biological Industries (Beit-Haemek, Israel). The quantitative polymerase chain reaction (qPCR) assay kits were acquired from Takara (Japan). SB202190, pyrrolidine dithiocarbamate (PDTC), SR11302, and N-acetyl-l-cysteine (NAC) were purchased from Selleck (Houston, USA), APExBIO (Houston, USA), or Beyotime Biotechnology (Shanghai, China). Fluorocitrate (FC) and GIBH-130 (GI) were bought from Sigma (New Jersey, USA) and MedChemExpress (New Jersey, USA), respectively. Short hairpin RNA (shRNA) targeting the interleukin-1β (IL-1β) gene was designed and synthesized by Hanbio (Shanghai, China), while small interfering RNAs (siRNAs) targeting the tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), or CYP2E1 genes were designed and synthesized by RiboBio (Guangzhou, China).

2.1. Reagents 2-Chloroethanol (2-CE) was obtained from Sinopharm Chemical Reagent Co., Ltd. (Ningbo, China), while reagents for the cell culture were purchased from Biological Industries (Beit-Haemek, Israel). The quantitative polymerase chain reaction (qPCR) assay kits were acquired from Takara (Japan). SB202190, pyrrolidine dithiocarbamate (PDTC), SR11302, and N-acetyl-l-cysteine (NAC) were purchased from Selleck (Houston, USA), APExBIO (Houston, USA), or Beyotime Biotechnology (Shanghai, China). Fluorocitrate (FC) and GIBH-130 (GI) were bought from Sigma (New Jersey, USA) and MedChemExpress (New Jersey, USA), respectively. Short hairpin RNA (shRNA) targeting the interleukin-1β (IL-1β) gene was designed and synthesized by Hanbio (Shanghai, China), while small interfering RNAs (siRNAs) targeting the tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), or CYP2E1 genes were designed and synthesized by RiboBio (Guangzhou, China).