Malvern mastersizer 3000e hydro

Static light scattering was used to measure the particle size distribution of the emulsion droplets and emulsion microgel particles via a Malvern Mastersizer 3000E hydro, (Malvern Instruments, Worcestershire, UK). Samples were diluted in distilled water until the instrument gave an obscuration of 4-6%. Sizing of the emulsion oil droplets was conducted based on a relative refractive index of 1.097 (i.e. the ratio of the refractive index of sunflower oil at 1.460 to that of the aqueous phase at 1.33). The absorbance value of the emulsion droplets was set to 0.001. Sizing of the emulsion microgel particles formed with Leeds Jet homogenizer was conducted based on a relative refractive index of 1.150 (i.e., the ratio of the refractive index of WPI at 1.53 to that of the aqueous phase at 1.33). The absorbance value of the emulsion microgel particles was similarly set to 0.001.
Emulsion microgel particles formed using the Buchi Encapsulator B-390 ® were sized using image analysis of the digitized images captured via a Nikon SMZ-2T
(Nikon, Japan) optical microscope, due to their larger sizes (> 500 µm). For comparison of particle size distributions the Sauter mean diameter (d32 ) and the De Brouckere mean diameter (d43 ) were calculated. Each sample was analysed ten times and the averages and standard deviations are reported.

Static light scattering was used to measure the size distribution of the emulsion droplets and emulsion microgel particles undergoing in vitro digestion (at 0, 5, 60, 120 min during gastric digestion; at 0, 30, 180 min during intestinal digestion; and at 0, 30, 180 min after gastric and during intestinal digestion) using a Malvern Mastersizer 3000E hydro, (Malvern Instruments, Worcestershire, UK). Samples were diluted in distilled water until the instrument gave an obscuration of 4 to 6%. Sizing of the emulsion oil droplets was conducted based on a relative refractive index of 1.097 (i.e., the ratio of the refractive index of sunflower oil at 1.460 to that of the aqueous phase at 1.33). The absorbance value of the emulsion droplets was set to 0.001. Sizing of the emulsion microgel particles was conducted based on a relative refractive index of 1.150 (i.e., the ratio of the refractive index of WPI at 1.53 to that of the aqueous phase at 1.33). The absorbance value of the emulsion microgel particles was similarly set to 0.001.
For comparison of particle size distributions the Sauter mean diameter ( d32) and the De Brouckere mean diameter (d43 ) were calculated. Each sample was analysed ten times and the averages and standard deviations are reported.