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1 2 dimyristoyl phosphatidylcholine

Manufactured by Avanti Polar Lipids
Sourced in United States

1,2-dimyristoyl phosphatidylcholine is a synthetic lipid compound commonly used in laboratory research. It is a phospholipid with two 14-carbon myristoyl fatty acid chains and a phosphatidylcholine polar headgroup. This lipid is widely employed as a model membrane system for biophysical and biochemical studies.

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2 protocols using 1 2 dimyristoyl phosphatidylcholine

1

Purification and Lipid Preparation for Biophysical Studies

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St I was purified from the sea anemone S. helianthus as previously described [1 (link)]. The lipids: 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC); egg sphingomyelin (eggSM); 1,2-dimyristoyl phosphatidylcholine (DMPC); 1,2-dipalmitoyl phosphatidylcholine (DPPC); 1,2-distearoyl phosphatidylcholine (DSPC); 1,2-diarachidoyl phosphatidylcholine (DAPC); 1,2-dioleoyl phosphatidylcholine (DOPC) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). The fluorescent probes carboxyfluorescein (CF), 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate (DiD), and Alexa 488, Alexa 555 C5 maleimide, Dextran 3000-Alexa 488 and Dextran 10000-Alexa 647 were obtained from Invitrogen (Eugene, OR, USA). Solvents and chemicals were of the highest commercial purity available.
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2

Liposomal Encapsulation of Pharmaceutical Agents

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Egg phosphatidylcholine (PC), 1,2-dimyristoylphosphatidylcholine (DMPC), 1,2diplmitoylphosphatidylcholine (DPPC), 1,2-disteroylphosphatidylcholine (DSPC) were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL). Cholesterol was from Sigma-Aldrich Company Ltd. (Poole, UK). Glipizide, metformin HCl and Phosphate buffered saline (PBS) in tablet form (pH 7.4) were purchased from Sigma-Aldrich Company Ltd. (Poole, UK). All the solvents used in the analysis were of analytical grade and were purchased from Fisher Scientific UK (Loughborough, UK). Water used in the process of liposome preparation was of milli-Q grade.
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