The HCT 116, HT-29, and KM12 cell lines were purchased directly from the National Institutes of Health, National Cancer Institute (NCI, Frederick, MD, USA); the SW480, SW403, SNU-C1, and SNU-C5 cell lines were purchased from the Korean Cell Line Bank (KCLB, Seoul, Korea); and the DLD-1 and HT-55 cell lines were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) and Sigma-Aldrich (St. Louis, MO, USA), respectively. DLD-1, HCT 116, HT-29, KM12, SW403, and SW480 cells were maintained in RPMI 1640 medium (HyClone Laboratories, Logan, UT, USA) containing 10% fetal bovine serum (FBS; HyClone Laboratories) and 1% penicillin/streptomycin (PS; Thermo Fisher Scientific, Waltham, MA, USA). HT-55 cells were maintained in MEM (HyClone Laboratories) containing 10% FBS (HyClone Laboratories) and 1% PS (Thermo Fisher Scientific). SNU-C1 and SNU-C5 cells were maintained in RPMI 1640 medium containing 25 mM HEPES (HyClone Laboratories), 10% FBS (HyClone Laboratories), and 1% PS (Thermo Fisher Scientific). All cell lines were maintained under a humidified atmosphere of 5% CO2 and 95% air at 37 °C. The culture media were refreshed every 2 to 3 days.
Fetal bovine serum (fbs)
Manufactured by Cytiva
Sourced in United States, China, Japan, United Kingdom, Australia, Canada, New Zealand, Israel, Germany, Sweden, India
FBS is a cell culture supplement produced by Cytiva. It is a complex mixture of proteins, growth factors, and other biological components that support the growth and maintenance of a wide range of cell types in vitro. FBS is commonly used in cell culture applications to provide essential nutrients and support for cell proliferation and differentiation.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (143 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (133 mentions)
Penicillin, by Thermo Fisher Scientific (112 mentions)
Streptomycin, by Thermo Fisher Scientific (111 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (85 mentions)
Penicillin streptomycin, by Cytiva (68 mentions)
Dulbecco modified eagle medium (dmem), by Cytiva (61 mentions)
L glutamine, by Thermo Fisher Scientific (57 mentions)
Streptomycin, by Cytiva (51 mentions)
Rpmi 1640 medium, by Cytiva (51 mentions)
Penicillin, by Cytiva (50 mentions)
Rpmi 1640, by Cytiva (37 mentions)
Dulbecco s modified eagle s medium dmem, by Cytiva (36 mentions)
Rpmi 1640, by Thermo Fisher Scientific (36 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (32 mentions)
Trypsin edta, by Thermo Fisher Scientific (30 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (29 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (28 mentions)
Glutamax, by Thermo Fisher Scientific (28 mentions)
Dulbecco s modified eagle s medium, by Cytiva (27 mentions)
1 307 protocols using fetal bovine serum (fbs)
1
Culture and Maintenance of Colorectal Cancer Cell Lines
2
Co-culture of H9c2 and Wharton's Jelly Stem Cells
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H9c2 cardiomyoblast cells (ATCC CRL-1446, USA) were cultured in Dulbecco’s modified Eagle Medium containing 10% fetal bovine serum (Cytiva, Marlborough, MA, USA) and 1% Antibiotic-Antimycotic under an incubator at 5% CO2 and 37 °C. Human Wharton’s jelly stem cells (WJSC, Tseng Hsiang Life Science Ltd., Taipei, Taiwan) were cultured in HiMesoXL Mesenchymal Stem Cell Expansion Medium (HiMedia Laboratories Pvt. Ltd., Thane West, Maharashtra, India) containing 10% fetal bovine serum (Cytiva, Marlborough, MA, USA) and 1% Antibiotic-Antimycotic under an incubator with 5% CO2 and 37 °C. When performing the co-culture, a hanging insert (PET, 0.4 μm, 24 wells, Merck KGaA, Darmstadt, Germany) was placed in the culture dish and used to separate the H9c2 cells (in a 10 cm culture dish with 80% confluency) and WJSC (in the insert with 5 × 105 cells) into different layers, and H9c2/WJSC co-culture was performed in Dulbecco’s Modified Eagle Medium containing 10% fetal bovine serum (Cytiva, Marlborough, MA, USA) and 1% Antibiotic–Antimycotic in an incubator at 5% CO2 and 37 °C.
3
Murine Myoblast C2C12 Cell Culture
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Murine myoblast C2C12 cells (Korean Cell Line Bank, Seoul, Korea) were cultured in DMEM (Dulbecco’s Modified Eagle’s Medium; HyClone, Logan, UT, USA) supplemented with 10% (for cell proliferation) or 2% (for cell differentiation) FBS (fetal bovine serum, Hyclone Laboratories) and 1% P/S (penicillin/streptomycin, Hyclone Laboratories, Logan, UT, USA). At 90–95% cell confluency, the cell culture medium was changed with a differentiation medium, and the media was replaced every 1–2 days.
4
Propagating and Maintaining TSA Mammary Carcinoma Cells
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TSA mammary carcinoma cells were propagated in DMEM GlutaMAXTM (Gibco) containing 4.5 g/L D-glucose and supplemented with 10% (v/v) fetal bovine serum (HyClone Laboratories). Prior to transfection or experimentation, TSA cells tested negative for Mycoplasma using a CELLshipper Mycoplasma Detection Kit (Bionique). All TSA derived clones were propagated and assayed in phenol-free DMEM supplemented with 10% (v/v) fetal bovine serum (HyClone Laboratories), 4.5 g/L D-glucose, L-glutamine, and 25 mM HEPES (Gibco). Additionally, all TSA derived clones were selected and maintained in 400 µg/mL G418 (Gibco). All cell lines were grown in a humidified incubator (ThermoScientific) at 37 °C and a 5% CO2 atmosphere.
5
Antimicrobial Evaluation of Bacterial Strains and Skin Cell Culture
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Escherichia coli CICC 10003, Staphylococcus aureus CICC 10384, Listeria monocytogenes CMCC 54004, Salmonella typhimurium CICC 21484 were purchased from the China Center of Industrial Culture Collection (Beijing). Listeria monocytogenes CMCC 54004 was obtained from the College of Food Science and Technology, Yunnan Agricultural University (Kunming, Yunnan Province, China). Bacterial strains were conserved at -80°C in glycerol containing nutrient broth and were subcultured twice in Luria-Bertani broth medium under agitation (100 rpm) at 37°C for 24 h before use. The initial concentration of cells was adjusted to 10 6 cfu/mL by dilution in PBS (pH 7.4, containing 137 mM NaCl, 2.7 mM KCl, 10.1 mM Na 2 HPO 4 , and 1.8 mM KH 2 PO 4 , without calcium and magnesium) for subsequent experiments.
Human skin epithelial cells (HaCaT) were provided by the Moringa Institute of Yunnan Agricultural University. Cells were stored with 90% fetal bovine serum and 10% dimethyl sulfoxide at -80°C and cultured in RPMI 1640 medium containing 10% heat-inactivated fetal bovine serum (Hyclone Laboratories, Logan, UT) and 1% penicillin streptomycin antibiotic mixture (Gibco, Waltham, MA). All cell lines were cultured at 37°C in a humidified atmosphere at 5% CO 2 and 95% air.
Human skin epithelial cells (HaCaT) were provided by the Moringa Institute of Yunnan Agricultural University. Cells were stored with 90% fetal bovine serum and 10% dimethyl sulfoxide at -80°C and cultured in RPMI 1640 medium containing 10% heat-inactivated fetal bovine serum (Hyclone Laboratories, Logan, UT) and 1% penicillin streptomycin antibiotic mixture (Gibco, Waltham, MA). All cell lines were cultured at 37°C in a humidified atmosphere at 5% CO 2 and 95% air.
6
Diosmetin and Diosmin Characterization
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Diosmetin and diosmin were purchased from ChemFaces (Wuhan, China), their purity was over 98%. 2,4-Dinitrochlorobenzene (DNCB) was obtained from Sigma-Aldrich Co (St. Louis, MO, USA). DMEM (Dulbecco’s Modified Eagle’s Media) and FBS (Fetal Bovine Serum) were obtained from HyClone Laboratories, Inc (Logan, UT, USA). Penicillin-streptomycin solution and Fast SYBR® Green Master Mix were purchased from Life Technologies (Waltham, MA, USA). An RNeasy mini kit and a ImProm-II Reverse Transcription System kit were purchased from Qiagen (Valencia, CA, USA) and Promega (Madison, WI, USA), respectively.
7
Isolation and Culture of PBMC and HaCaT Cells
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Healthy peripheral blood mononuclear cells were separated over Ficoll-Hypaque gradients (MP Biomedicals, Aurora, OH, USA). PBMC were grown in RPMI 1640 (Invitrogen, San Diego, CA, USA), supplemented with 2 mM l -glutamine, 50 ng/mL, streptomycin, 50 units/mL penicillin, and 10% heat-inactivated fetal bovine serum (Hyclone Laboratories, Logan, UT, USA). All volunteers provided written informed consent in agreement with the Declaration of Helsinki to the use of their residual buffy coats for research aims with approval from the University Hospital of Salerno Review Board.
Human immortalized keratinocytes (HaCaT) were grown in Dulbecco’s modified Eagle’s medium (DMEM, GIBCO, Grand Island, NY, USA) supplemented with 2 mMl -glutamine, 50 ng/mL, streptomycin, 50 units/mL penicillin, and 10% heat-inactivated fetal bovine serum (Hyclone Laboratories, Logan, UT, USA). HaCaT cells were kindly provided by Giuseppe Monfrecola (Department of Experimental Dermatology, University of Naples, Naples, Italy).
Human immortalized keratinocytes (HaCaT) were grown in Dulbecco’s modified Eagle’s medium (DMEM, GIBCO, Grand Island, NY, USA) supplemented with 2 mM
8
Adipogenic Differentiation of hMSCs and HUVECs
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hMSCs and HUVECs were obtained from American type culture collection (ATCC, Manassas, VA). DMEM (Dulbecco’s modified Eagle medium), EDTA (ethylenediaminetetraacetic acid), and trypsin were purchased from Gibco (Paisley, United Kingdom). FBS (fetal bovine serum) and penicillin-streptomycin were obtained from Hyclone Laboratories, U.S. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], propidium iodide, JC-1 stain, Oil Red O, Nile red, and all other chemicals related to molecular biology experiments were purchased from Sigma-Aldrich (St. Louis, MO).
Adipocyte differentiation factors, 3-isobutyl-1-methyl-xanthine (IBMX), rosiglitazone, dexamethasone (DEX), and insulin were purchased from Sigma (St. Louis, MO). The cell to cDNA synthesis kits and SYBR Green PCR master mix were obtained from Qiagen (Hilden, Germany). ELISA kits for proinflammatory cytokine were purchased from Qiagen (Hilden, Germany).
Adipocyte differentiation factors, 3-isobutyl-1-methyl-xanthine (IBMX), rosiglitazone, dexamethasone (DEX), and insulin were purchased from Sigma (St. Louis, MO). The cell to cDNA synthesis kits and SYBR Green PCR master mix were obtained from Qiagen (Hilden, Germany). ELISA kits for proinflammatory cytokine were purchased from Qiagen (Hilden, Germany).
9
Culturing Murine C2C12 Myoblasts
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Murine C2C12 myoblast cells (Korean Cell Line Bank, Seoul, Korea) were cultured in growth media [DMEM (HyClone Laboratories, South Logan, UT, USA) + 10% FBS (fetal bovine serum, HyClone Laboratories) + 1% P/S (Penicillin/Streptomycin, Thermo Fisher Scientific, Waltham, MA, USA)] in a humidified 5% CO2 incubator at 37 °C.
10
Cell Culture Protocol for TS/A and 4T1 Cells
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