T24 cell line

The human bladder cancer T24 cell line was obtained from China Center for Type Culture Collection. T24 cells were cultured in MEM medium (Hyclone) with 10% fetal bovine serum (Gibco) and 1% penicillin-streptomycin (Hyclone) and maintained in 37°C with 5% CO₂ incubator. The sequence for the sh-RNAs targeting SEC23A was 5′-GGAAGCTACAAGAATGGTTGT-3′. The lentivirus particles of shSEC23A were prepared by Sangon Biotech Co.

Human bladder cancer T24 cell line was purchased from the China Center for Type Culture Collection (Wuhan University Collection Center, Wuhan, China). Human bladder cancer cell line EJ and human bladder immortalized epithelium cell line SV-HUC-1 were kindly provided by Associate Professor Huageng Liang, Department of Urology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, China. Human normal liver cell line L02 was purchased from iCell Bioscience Inc. (Shanghai, China). Human embryonic kidney cell line HEK293T was obtained from American Type Culture Collection (ATCC). Human lung cancer A549, cervical cancer Hela, glioma cancer U87, breast cancer MCF-7 and MDA-MB-231, and prostate cancer PC-3 cell lines were obtained from the College of Life Science and Technology, Huazhong University of Science and Technology (Wuhan, China). T24, EJ, A549, MCF-7, and U87 cells were cultured in Eagle’s minimum essential medium (MEM) supplemented with 10% fetal bovine serum (FBS). L02, HEK293T, Hela, MDA-MB-231, and PC-3 cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS). SV-HUC-1 cells were grown in Ham’s F-12K medium supplemented with 10% FBS. All the cultures were maintained at 37°C in a humidified 5% CO₂ incubator.