Amyloglucosidase e amgdf

The mung bean variety (JLV 8) was purchased from Gucheng Sandou Food Technology Development Co. Ltd., (Heng Shui, Hebei Province, China). Salivary α-amylase (A-3176, Sigma, St. Louis, MO, USA), pepsin (P-6887, Sigma St. Louis, MO, USA), porcine pancreatic enzymes (P-1750, Sigma), amyloglucosidase (E-AMGDF, 3300 U/mL, Megazyme, Bray, Ireland) were used for starch digestion. D-Glucose (k-Gluc, GOPOD format, Megazyme, Bray, Ireland) was used for the glucose measurements. All other reagents used in experiments were of the analytical grade.

The fruit of the Chinese chestnut variety (Yanshan Zaofeng), one of the chestnut varieties with the largest yield and the best flavor in the Yanshan region, were collected from the Chestnut Industry Collaborative Innovation Center of Hebei province, China, in the 2020 season. The fruit were stored at 4 °C before analysis. Chestnut starch was obtained through the alkaline method following the procedures of our previous report [20 (link)]. The chestnut starch had a high purity of >98%, with 0.13% ash, 0.23% protein and 0.80% lipid.
A glucose oxidase/peroxidase (GOPOD) kit and amyloglucosidase (E-AMGDF, 3300 U/mL) were provided by the Megazyme International Ireland Ltd. (Bray Co., Wicklow, Ireland). Amylose (A0512) and amylopectin (A8515) from potato starch were purchased from Sigma Chemical Co. (St. Louis, MO, USA).