Epr19523

Manufactured by Abcam

EPR19523 is a recombinant monoclonal antibody that recognizes Phospho-Tau (Thr181). It is intended for use in research applications.

Automatically generated - may contain errors

Lab products found in correlation

Mops sds running buffer, by Thermo Fisher Scientific (1 mentions) Ab92544, by Abcam (1 mentions) Ab16080, by Abcam (1 mentions) Ep2000y, by Abcam (1 mentions) Mes sds running buffer, by Thermo Fisher Scientific (1 mentions) Ibright system, by Thermo Fisher Scientific (1 mentions)

2 protocols using epr19523

Western Blotting of Alzheimer's Proteins

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Samples were loaded onto 4–12% or 8% Bis-Tris gels using MES-SDS or MOPS-SDS running buffer (Invitrogen), transferred to nitrocellulose membranes, and probed for various proteins using standard WB. The resultant blots were detected with ECL and exposure to film. The antibodies used to detect specific antigens were, for mBACE1, EPR19523 (rabbit; Abcam); for hBACE1, D10E5 (rabbit, CST, RRID: AB_1903900); for ADAM10, MABN1123 (rabbit, RRID: AB_10972023; Abcam); for MT5-MMP, GTX128246 (rabbit; Genetex); for Meprinβ, PA5-47474 (rabbit, RRID: AB_2607364; Thermo Fisher Scientific); for PS1-N, SC-7860 (rabbit, RRID: AB_2170581; Santa-Cruz); for PS1-C, EP-2000Y (rabbit, RRID: AB_1310605; Abcam); for PS2-C, EP-1515Y (rabbit, RRID: AB_882202; Abcam); for NCT, SC-14369 (goat, RRID: AB_2282503; Santa-Cruz); for Aph-1aL, PRB-550P (rabbit, RRID: AB_291682; BioLegend): for SPP, ab16080 (rabbit, RRID: AB_302242; Abcam); for SPPL2b, PA5-42683 (rabbit, Abcam RRID: AB_2606446; Abcam); for APP-CTF, C-7 (rabbit, homemade); for APP (573–596), SIG-39180 (mouse, RRID: AB_10719734; BioLegend); for mAPP (597), 28055 (rabbit, RRID: AB_918338; IBL); for APP-CTF (asp1), 3D6 (mouse, homemade); for LRP1, ab92544 (rabbit, RRID: AB_2234877; Abcam); for APLP1, W1CT (rabbit, homemade); and for RHBDL2, 12467-1-AP (rabbit, RRID: AB_11232403; Proteintech).

Liu L., Ding L., Rovere M., Wolfe M.S, & Selkoe D.J. (2019). A cellular complex of BACE1 and γ-secretase sequentially generates Aβ from its full-length precursor. The Journal of Cell Biology, 218(2), 644-663.

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Western Blot Analysis of BACE1, SNAP-25, and Tubulin

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Samples were loaded onto 4–12% Bis-Tris gels using MES-SDS running buffer (Invitrogen), transferred to PVDF membranes, and probed for various proteins using standard WB. The resultant blots were detected by ECL and signals were captured by the iBright system (Invitrogen) or film. The antibodies used to detect specific antigens were: for mouse BACE1, EPR19523 (rabbit, Abcam); for human BACE1, MAB5308, (mouse, Millipore, RRID: AB_95207); for SNAP-25, 610366, (mouse, BD, RRID: AB_397752); and for α-tubulin, T5168 (mouse, Sigma, RRID: AB_477579).

Liu L., Lauro B.M., Ding L., Rovere M., Wolfe M.S, & Selkoe D.J. (2019). Multiple BACE1 Inhibitors Abnormally Increase the BACE1 Protein in Neurons by Prolonging its Half-life. Alzheimer's & dementia : the journal of the Alzheimer's Association, 15(9), 1183-1194.

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