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Pseudomonas agar base with cn supplement

Manufactured by Thermo Fisher Scientific

Pseudomonas agar base with CN supplement is a microbiological culture medium used for the isolation and enumeration of Pseudomonas species from clinical and non-clinical samples. The supplement provides selective inhibition of Gram-positive bacteria, allowing for the growth of Pseudomonas species.

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2 protocols using pseudomonas agar base with cn supplement

1

Isolation and Identification of Enterobacteriaceae and Pseudomonas aeruginosa

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A loopful of all the previously inoculated broths was cultured on eosin methylene blue (EMB) agar (Oxoid, Oxoid Ltd., London) for isolation of Enterobacteriaceae other than salmonellae. Pseudomonas agar base with CN supplement (Oxoid) was used for the selective isolation of P. aeruginosa. All the inoculated plates were incubated aerobically at 37 °C for 24 h. Suspected colonies were picked and examined for morphological and culture characteristics according to Quinn et al. [20 ].
The API 20E and API 20NE kits (BioMerieux, France) were used according to the manufacturer’s instructions to detect the biochemical profiles of the isolated Enterobacteriaceae and P. aeruginosa, respectively.
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2

Microbial Isolation and Quantification from Combat Wounds

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CA and two different strains of P. aeruginosa isolated from clinical combat wounds (PA1 and PA2, obtained from the US Army Institute of Surgical Research, Fort Sam Houston, TX, USA) were used. Tryptic soy broth (TSB) was used as the growth medium for both microorganisms. For quantification, Pseudomonas Agar Base with CN supplement (Oxoid) was used to isolate P. aeruginosa and BBL CHROMagar Candida was used to isolate C. albicans.
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