Elisa kits for human fgf21

Blood samples were collected on admission to the hospital for patients with AMI or at the time of scheduled admission for the stable AP patients who undergo elective PCI. After samples were immediately processed to separate serum and were used for quantitative determination of conventional laboratory metabolites (LDL-cholesterol, HDL-cholesterol, triglyceride, glucose), serum was stored at −30 °C until further analysis. Concentrations of FGF21 and FABP4 were quantified by using commercially available enzyme-linked immunosorbent assay (ELISA) kits for human FGF21 (R&D Systems) and human FABP4 (Biovendor), respectively. As specified by the manufacturer, the lower limit of detection of serum FGF21 was 4.67 pg/mL and FABP4 was 0.05 ng/mL. cTnT concentrations were determined with the use of high-sensitivity electrochemiluminescence assays in the Elecysy-2010 system (Roche Diagnostics). FFA concentrations were determined by enzyme-linked assays and individual fatty acids were analyzed by capillary gas chromatography at SRL Inc., and were expressed as a percentage of total fatty acids. We evaluated the time course of the changes in the circulating FGF21, FABP4, FFA, and cTnT levels in a subgroup of AMI patients whose blood samples could be serially collected on admission, immediately after emergent PCI, at 3, 6 h and 1 day after PCI.

The protein levels of FGF21 in HCF culture supernatant were quantified using an enzyme-linked immunosorbent assay (ELISA) kits for human FGF21 (R&D Systems, Minneapolis, MN, USA), according to the manufacturer’s instructions.