Balb c rag2 mice

All mice used in this study were bred in the animal facility of the Brussels branch of the Ludwig Institute for Cancer Research under specific pathogen-free conditions. Rag2^−/− BALB/c mice were originally purchased from Taconic and C57BL/6NJ Il24^−/− mice were purchased from Jackson laboratory. Il20rb^−/− mice, in C57BL/6 background, were provided by U.M. Wegenka (University Medical Center, Ulm, Germany)^{25 (link)}. Wild-type (WT) 129/Sv mice were originally purchased from Harlan. Il22–deficient mice were generated in 129/Sv background in our laboratory as described previously^{44 (link)}. IL-22R-deficient mice were generated in 129/Sv and C57BL/6 background in our laboratory as described in Suppl. Fig. 7. All mice were bred as heterozygous and littermate controls were used for in vivo experiments. The Il22^−/− mice were used in 129/Sv background, Il22ra1^−/− mice were used in 129/Sv or C57BL/6 background and Il24^−/− and Il20rb^−/− in C57BL/6 background. The experiments were performed in compliance with institutional guidelines and were approved by the Animal Research Ethical Committee of the Université catholique de Louvain (2015/UCL/MD/09). Mice between 8 and 12 weeks of age were shaved on the back skin one day before CHS triggering.

BALB/c WT and BALB/c nu/nu mice were obtained from Japan SLC, Inc. DO11.10 transgenic BALB/c mice whose T cells express an OVA-specific receptor were kindly provided by T. Watanabe (Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan). Rag2^−/− BALB/c mice and TCRα^−/− BALB/c mice were purchased from Taconic Farms. Scurfy C57BL/6 mice and Thy1.1⁺ BALB/c mice were obtained from the Jackson Laboratory. Ld-nOVA transgenic mice were generated as previously reported46 (link), and were backcrossed to the BALB/c background for 8 generations and intercrossed, and Ld-nOVA BALB/c mice were subsequently maintained as homozygotes. Mice were bred in our facility under specific pathogen-free conditions. Female, age-matched (6–10 weeks-old at the start) mice were used in all experiments. The animal care and procedures of the experiments were approved by the Ethical Committee on Animal Experiments of the University of Tokyo, and all experiments were performed in accordance with the institutional and national guidelines.