Experiments were performed as described1 (link). Fat tissues were grounded in RIPA buffer [50 mM Tris pH 7.5, 1 % NP40, 0.5 % sodium deoxycholate, 0.1 % SDS, 150 mM NaCl, 5 mM EDTA, and protease inhibitor cocktail (45 μg/ml, Roche, 11 873 580 001)] at 4 °C. Homogenates were separated in 10 % polyacrylamide gels and blotted to Hybond nitrocellulose membranes (GE Healthcare). Membranes were decorated using following antibodies: anti-LSD11 (link) (1/1000), anti-Prdm16 (abcam, ab118573, 1/500), anti-Ucp1 (abcam, ab10983, 1/1000), Fabp4 (Santa-Cruz, sc-18661, 1/2000), anti-Nrf1 (abcam, ab55744, 1/1000), anti-Flag (Sigma, F3165, 1/500), anti-β-Tubulin (Sigma, T6074, 1/10000), or anti-β-Actin (Sigma, A1978, 1/10000). Secondary antibodies conjugated to horseradish peroxidase (GE Healthcare) were detected using an enhanced chemiluminescence detection system (GE Healthcare). Protein levels were quantified using the Chemi Capt software (Peqlab). Uncropped scans are available in Supplementary Fig. 16 .
Sc 18661
Manufactured by Abcam
Sc-18661 is a product offered by Abcam, a leading provider of high-quality reagents and tools for researchers. This product is a primary antibody, but detailed information about its specific function or intended use is not available.
Automatically generated - may contain errors
Lab products found in correlation
Ab10983, by Santa Cruz Biotechnology (2 mentions)
Ab118573, by Abcam (2 mentions)
Ab55744, by Merck Group (2 mentions)
Horseradish peroxidase, by GE Healthcare (2 mentions)
Enhanced chemiluminescence detection system, by GE Healthcare (2 mentions)
A1978, by Merck Group (2 mentions)
Hybond nitrocellulose membrane, by GE Healthcare (2 mentions)
Chemi capt software, by Avantor (2 mentions)
T6074, by Merck Group (2 mentions)
F3165, by Merck Group (2 mentions)
Anti β actin, by Merck Group (2 mentions)
Protease inhibitor cocktail, by Roche (2 mentions)
2 protocols using sc 18661
1
Immunoblotting Analysis of Adipose Tissue
2
Immunoblotting Analysis of Adipose Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Experiments were performed as described1 (link). Fat tissues were grounded in RIPA buffer [50 mM Tris pH 7.5, 1 % NP40, 0.5 % sodium deoxycholate, 0.1 % SDS, 150 mM NaCl, 5 mM EDTA, and protease inhibitor cocktail (45 μg/ml, Roche, 11 873 580 001)] at 4 °C. Homogenates were separated in 10 % polyacrylamide gels and blotted to Hybond nitrocellulose membranes (GE Healthcare). Membranes were decorated using following antibodies: anti-LSD11 (link) (1/1000), anti-Prdm16 (abcam, ab118573, 1/500), anti-Ucp1 (abcam, ab10983, 1/1000), Fabp4 (Santa-Cruz, sc-18661, 1/2000), anti-Nrf1 (abcam, ab55744, 1/1000), anti-Flag (Sigma, F3165, 1/500), anti-β-Tubulin (Sigma, T6074, 1/10000), or anti-β-Actin (Sigma, A1978, 1/10000). Secondary antibodies conjugated to horseradish peroxidase (GE Healthcare) were detected using an enhanced chemiluminescence detection system (GE Healthcare). Protein levels were quantified using the Chemi Capt software (Peqlab). Uncropped scans are available in Supplementary Fig. 16 .
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