Mouse ast kit

CA, CCl₄, GW4064, olive oil, and EE2 were purchased from Sigma-Aldrich. 1,2-Propanediol and corn oil were purchased from Acros Organics. Mouse TBA kits were purchased from Crystal Chem. Mouse AST Kit was purchased from Biovision.

All recruited pregnant women had baseline liver function tests performed at the first antenatal visit at gestation weeks 12–14, followed by at least one subsequent testing at gestation weeks 24–28. Additional testing was requested as clinically appropriate at gestation weeks 32–36. Serum samples were collected from pregnant women after fasting for at least 8 h. sTBA, AST, ALT, GGT, and TBiL levels were measured using an automatic biochemical analyzer (AU2700, Olympus, Japan). The highest values among the 2–3 tests for each individual subjects were used for the data analyses. Mouse sTBAs were quantified with the mouse TBA Kit from Crystal Chem. Mouse AST assays were carried out with the Mouse AST Kit from Biovision.