Bio plex protm mouse cytokine th17 panel a 6 plex kit

Murine serum was carefully collected from whole blood and stored in −80°C. Wound edge tissue was harvested after the mice were sacrificed, washed twice in distilled PBS, and cut into small pieces in lysis buffer containing protease inhibitor and 0.1 mM PMSF (Sangon Biotech, Shanghai, China), followed by homogenization and centrifugation (12,000 rpm, 20 min). The supernatant was then collected for further assessment of EGF (MM-0043M), bFGF (MM-0050M1), PDGF (MM-0070M1), and TGF-β1 (MM-0921M) levels using mouse ELISA kits (Jiangsu Meimian industrial, China). Tissue and serum samples underwent multiple cytokines detection by using Bio-plex pro^TM Mouse Cytokine Th17 Panel A 6-Plex kit (#M6000007NY, Bio Rad, USA). For tissue detection, amounts of target growth factors were normalized to the total amount of whole protein.

Murine serum was carefully collected from the other half of the whole blood after a routine test and stored at −80 °C. Serum samples underwent multiple cytokine detection by using the Bio-plex pro-TM Mouse Cytokine Th17 Panel A 6-Plex kit (#M6000007NY, Bio Rad, Hercules, CA, USA).