Q150r es coater

The characterization of surface morphology of microencapsulated fish oils was done using scanning electron microscope (SEM). Microencapsulated fish oil samples were coated with gold for 30 s to a thickness of 2 nm using a Q150R ES Coater (Quorum Technologies, UK). The coated samples were observed using the SEM (Quanta FEG650, USA) at voltage of 20 kV with a magnification of 1,000 and 5,000 and working distance of 10.8–10.9 mm. Carbon tape is used for the adhesion of the material and for conductivity. Morphological structure of microcapsules was characterized by a scanning electron microscope (SEM) in Central Research Laboratory of Cukurova University (CUMERLAB, Adana, Türkiye). The particle sizes of the microencapsulates were also measured using SEM.

Images of the non-woven mats were collected using scanning electron microscope Vega 3 SEM (Tescan, Brno, Czech Republic) equipped with a secondary electron detector (SE) and backscattered electron detector (BSE). To visualize the entire surface the magnifications of 50× and 1000× were selected. The prepared sample was placed onto an aluminum specimen mount with carbon tape; after that, a thin gold layer was sputtered onto the sample surface using Q150R ES coater (Quorum, Laughton, UK) to prevent charging of the specimen. All measurements were performed using ImageJ software from National Institutes of Health (v.1.8.0_172, Bethesda, MD, USA).

The morphology of the particles obtained through spray drying was characterized using Quanta 650 field emission scanning electron microscopy (SEM, FEI Company, Hillsborn, OR, USA) at Cukurova University Central Research Laboratory (CUMERLAB, Adana, Turkey). SEM images were obtained at room temperature and 20 kV voltage, and the results were visually recorded at 10,000 and 20,000 magnification after gold coating with a Q150R ES Coater (Quorum Technologies, Lewes, UK).