Vanillin

Manufactured by Sangon

Sourced in China

Vanillin is a chemical compound that is the primary component responsible for the flavor and aroma of vanilla. It is commonly used in the food, fragrance, and pharmaceutical industries.

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Lab products found in correlation

Acetic acid, by Guangzhou Chemical (3 mentions) Sodium hydroxide (naoh), by Guangzhou Chemical (3 mentions) Acetonitrile, by Tianjin Damao (3 mentions) Na2hpo4, by Guangzhou Chemical (3 mentions) Citric acid, by Guangzhou Chemical (3 mentions) Hydrochloric acid (hcl), by Guangzhou Chemical (3 mentions) Sodium citrate tribasic dihydrate, by Merck Group (1 mentions) N hexane, by Chron Chemicals (1 mentions) Millipore q purification system, by Merck Group (1 mentions) Glycine, by Sinopharm (1 mentions) Citric acid, by Sangon (1 mentions) Sodium hydroxide (naoh), by Sangon (1 mentions) Tetracycline tc, by Merck Group (1 mentions) Nalidixic acid, by Merck Group (1 mentions) 4 hydroxybenzaldehyde, by Sangon (1 mentions)

4 protocols using vanillin

Vanillin biodegradation by activated sludge

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Vanillin was purchased from Sangon Biotech (Shanghai, China). The mixed culture was obtained from the activated sludge of a municipal wastewater treatment plant (Beijing, China), and analyzed by a MiSeq system of Majorbio (Shanghai, China).
The activated sludge was washed three times with phosphate buffer solution (PBS) to remove EPSs and organic matters in the sludge solution, and resuspended in synthetic wastewater to prepare a mixed bacterial suspension (100 mg VSS/L) (approximately 10 8 CFU/mL). The synthetic wastewater contains (mg/L): chemical oxygen demand (COD, calculated as glucose) 825; NH4Cl 192; KH2PO4 35.1; NaCl 100; MgSO4 100; CaCl2 10; pH = 6.5-7.5.

Si X., Ding D., Jian-hua Z., & Cao Z. (2020). Inhibitory Effect of Vanillin on Biofilm Formation by Multi-Species Wastewater Culture. Journal of New Materials for Electrochemical Systems, 23(4), 274-279.

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Detailed Reagent Procurement Protocol

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Vanillin was purchased Shanghai Sangon Biological Engineering Technology (Shanghai, China). o-Toluidine and N, N-dimethylformamide were purchased from Shanghai Aladdin Bio-Chem Technology Co., LTD (Shanghai, China). Sodium citrate tribasic dihydrate and Tris were purchased from Sigma-Aldrich (Steinheim, Germany). Glycine was purchased from GenStar Biosolutions Co., Ltd. (Beijing, China). Citric acid, disodium hydrogen, HCl, NaOH, Na₂HPO₄ and acetic acid were purchased from Guangzhou Chemical Reagent Factory (Guangzhou, China). Acetonitrile was purchased from Tianjin Damao Chemical Reagent Factory (Tianjin, China). n-Hexane was purchased from Chengdu Kelong Chemical Reagent Factory (Chengdu, China). Other chemicals were purchased from standard commercial sources and were of analytical grade purity.

Zhao J., Xia H., Yu T., Jin L., Li X., Zhang Y., Shu L., Zeng L, & He Z. (2018). A colorimetric assay for vanillin detection by determination of the luminescence of o-toluidine condensates. PLoS ONE, 13(4), e0194010.

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Preparation of Analytical Reagents

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Citric acid, NaOH, vanillin, d-(−)-salicin and glutathione were purchased from Sangon Biotech (Shanghai, China) Co., Ltd. Glucose and glycine were obtained from Sinopharm Group Chemical Reagent Co., Ltd (Shanghai, China). Sucrose was purchased from Shanghai Chemical Reagents Co., Ltd (Shanghai, China). Sodium dihydrogen phosphate dihydrate (NaH₂PO₄·2H₂O), disodium hydrogen phosphate dodecahydrate (Na₂HPO₄·12H₂O) and potassium hydrogen phosphate (K₂HPO₄·3H₂O) were bought from Tianjin Kemeiou Chemical Reagent Co., Ltd (Tianjin, China). Ascorbic acid was obtained from Shanghai Tri-Four Hewei Chemical Co., Ltd (Shanghai, China). Quinine sulfate was obtained from Tianjin Heowns Biochem Co., Ltd (Tianjin, China). All chemicals were of analytical grade and the water was distilled and passed through a Millipore Q purification system (Millipore Corporation).

Zhu S., Bai X., Wang T., Shi Q., Zhu J, & Wang B. (2021). One-step synthesis of fluorescent graphene quantum dots as an effective fluorescence probe for vanillin detection. RSC Advances, 11(16), 9121-9129.

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Construction of Z. mobilis Recombinants

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Zymomonas mobilis ZM4 (ATCC 31821) was purchased from American Type Culture Collection (Manassas, VA, USA). Shuttle vector pHW20a was used for construction of Z. mobilis recombinants [15 (link)]. Yeast extract was purchased from Oxoid (Hampshire, UK). Tetracycline (Tc) and nalidixic acid were from Sigma-Aldrich, St. Louis, MO, USA. 4-Hydroxybenzaldehyde, syringaldehyde, and vanillin were from Sangon Biotech Co., Shanghai, China. All other analytical grade chemicals were from Sinopharm Chemical Reagents, Shanghai, China.

Yi X., Gu H., Gao Q., Liu Z.L, & Bao J. (2015). Transcriptome analysis of Zymomonas mobilis ZM4 reveals mechanisms of tolerance and detoxification of phenolic aldehyde inhibitors from lignocellulose pretreatment. Biotechnology for Biofuels, 8, 153.

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