Activin-A was measured in undiluted decidual culture supernatants (n = 11) using the human activin-A duoset ELISA kit (R&D Systems) as per the manufacturer’s protocol. The concentrations were calculated in picogram per milliliter and corrected for the number of live cells per treatment group. Total STAT3 was measured in decidual cell lysates (n = 8) using the human STAT3 ELISA kit (Abcam ab176666) as per the manufacturer’s protocol. Cell lysates were prepared from 2 × 106 cells per treatment group and were in the range of 100–500 µg/ml of total protein. Lysates were aliquoted and stored at −80°C and assayed within a month. Total STAT3 concentrations were calculated in microgram per milliliter, corrected for total protein concentration per treatment group, and normalized to the control, which was set at 100%.
Human activin a duoset elisa kit
Manufactured by R&D Systems
The Human activin-A duoset ELISA kit is a tool used to detect and quantify the presence of the protein activin-A in human samples. It is a sandwich enzyme-linked immunosorbent assay (ELISA) that utilizes two specific antibodies to capture and detect the target analyte. The kit provides the necessary reagents and instructions to perform this assay.
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Lab products found in correlation
2 protocols using human activin a duoset elisa kit
1
Decidual Cell Activin-A and STAT3 Analysis
2
Quantification of Activin A in Cell Clusters
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Conditional media from the cell clusters isolated from 4‐week‐old control or KC mice were used for mouse cytokines analysis using RayBio Mouse Biotin‐Label Based Antibody Array (Mouse L‐308 Array, Glass Slide). For mouse Activin A quantification, Activin A in conditional media or serum was detected using the Human/Mouse/Rat Activin A Quantikine ELISA Kit (R&D Systems #DAC00B). For human Activin A quantification, Activin A in HPDE conditional media was detected using Human Activin a DuoSet ELISA Kit (R&D Systems #DY338). The amount of Activin A was normalized to the total protein in conditional media or sera and represented as pg mg−1.
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