Anti tlr 7 pe

Flow cytometry was employed to examine the immunophenotype of lymphocytes found in peripheral blood. The process involved obtaining a whole blood sample, which was then treated with a set of monoclonal human antibodies comprising anti-CD4 BV421, anti-CD3 PerCp, anti-CD8 BV605, anti-CD19 FITC, anti-CD45 Alexa Fluor 700, anti-CD56 BV650, anti-CD16 BV650, anti-TLR-2 APC, anti-TLR-4 PE, anti-TLR-7 PE, anti-TLR-8 APC, anti-TLR-3 PE, and anti-TLR-9 APC (Biolegend, San Diego, CA, United States). Following the antibody staining, a lysing buffer (BD, Franklin Lakes, NJ, United States) was applied to eliminate red blood cells, and the resulting cells were thoroughly washed and evaluated using a CytoFLEX LX instrument (Beckman Coulter, Indianapolis, IN, United States). Subsequently, data analysis was carried out utilizing the Kaluza Analysis program (sample analysis in Figure 1—CLL and Figure 2—CVID) The CytoFLEX LX flow cytometer was internally quality controlled using CytoFLEX Ready to Use Daily QC Fluorosphere reagents (Beckman Coulter, Indianapolis, IN, United States).

The analysis of lymphocyte immunophenotype in peripheral blood was performed through the use of flow cytometry, a precise and accurate approach to cell analysis. A whole blood sample was collected and treated with a set of monoclonal human anti-bodies consisting of anti-CD45 AF700, anti-CD3 PerCp, anti-CD4 BV421, anti-CD8 BV605, anti-CD19 FITC, anti-CD56 BV650, and anti-CD16 BV650, as well as anti-TLR2 APC, anti-TLR3 PE, anti-TLR4 PE, anti-TLR7 PE, anti-TLR8 APC, and anti-TLR9 APC antibodies (BioLegend, San Diego, CA 92121, USA). Subsequently, a lysing buffer was utilized to remove any red blood cells, and the remaining cells were thoroughly washed and assessed through the use of a CytoFLEX LX instrument, which is a sophisticated flow cytometer (Beckman Coulter, Indianapolis, IN, USA). The resulting data were analyzed using the Kaluza Analysis program, as demonstrated in Figure 2. The CytoFLEX LX flow cytometer was subjected to daily quality control using CytoFLEX Ready to Use Daily QC Fluorospheres reagents (Beckman Coulter, Indianapolis, IN, USA).